Observatorio de I+D+i UPM

Memorias de investigación
Communications at congresses:
Morphometric and genetic changes in chrysanthemum plants derived from cryopreservation
Year:2011
Research Areas
  • Crop production
Information
Abstract
There is some concern about the possible occurrence of somaclonal variation induced by cryopreservation techniques in plants. Morphological variations have been reported after in vitro culture and after cryopreservation. Recent genetic stability studies in cryopreserved chrysanthemum shoot apices have revealed that variation in the regenerated cryopreserved material is not a rare phenomenon and also that the instability can be observed in regenerants from certain pre-culture steps (Martín et al. 2011). Sixteen plants derived from several steps of an encapsulation-dehydration protocol were potted, grown in a green house for two years and used to study their morphological characters and genetic profiles by RAPD markers. Leaf (6), stem (1) and inflorescence/flowers (16) morphometric characters were studied and twelve RAPD primers were employed. Two of the cryopreserved samples showed morphometric differences with respect to other samples according to the principal component and cluster analyses performed, and differences in the RAPD profiles were found in some samples, although these two types of variations were not related.
International
Si
Congress
The Society for Low Temperature Biology Symposium 2011 "Preservation of Forest Species"
960
Place
Londres (Reino Unido)
Reviewers
No
ISBN/ISSN
Start Date
06/10/2011
End Date
07/10/2011
From page
To page
Participants
  • Autor: Y. Cifuentes (UPM)
  • Autor: Juan Bautista Martinez Laborde (UPM)
  • Autor: Maria Carmen Martin Fernandez (UPM)
  • Autor: M. Elena Gonzalez Benito (UPM)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Biodiversidad y conservación de recursos fitogenéticos
  • Departamento: Biología Vegetal
S2i 2020 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
Cofinanciación del MINECO en el marco del Programa INNPACTO (IPT-020000-2010-22)