Observatorio de I+D+i UPM

Memorias de investigación
Communications at congresses:
Processing Pipeline for Digitalizing the Lineage Tree of Early Zebrafish Embryogenesis from Multiharmonic Imaging
Year:2011
Research Areas
  • Medical equipment,
  • Electronic technology and of the communications,
  • Electronics engineering
Information
Abstract
The reconstruction of the cell lineage tree of early zebrafish embryogenesis requires the use of in-vivo microscopy imaging and image processing strategies. Second (SHG) and third harmonic generation (THG) microscopy observations in unstained zebrafish embryos allows to detect cell divisions and cell membranes from 1-cell to 1K-cell stage. In this article, we present an ad-hoc image processing pipeline for cell tracking and cell membranes segmentation enabling the reconstruction of the early zebrafish cell lineage tree until the 1K-cell stage. This methodology has been used to obtain digital zebrafish embryos allowing to generate a quantitative description of early zebrafish embryogenesis with minute temporal accuracy and ?m spatial resolution
International
Si
Congress
IEEE International Symposium on Biomedical Imaging: From Nano to Macro (ISBI 2011)
960
Place
Chicago, IL USA
Reviewers
Si
ISBN/ISSN
1945-7936
10.1109/ISBI.2011.5872699
Start Date
30/03/2011
End Date
02/04/2011
From page
1561
To page
1564
IEEE International Symposium on Biomedical Imaging: From Nano to Macro (ISBI 2011)
Participants
  • Autor: Miguel Angel Luengo Oroz (UPM)
  • Autor: José Luis Rubio Guivernau (UPM)
  • Autor: Maria Jesus Ledesma Carbayo (UPM)
  • Autor: Andres de Santos Lleo (UPM)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Tecnología de imágenes biomédicas
  • Centro o Instituto I+D+i: Centro de tecnología Biomédica CTB
  • Departamento: Ingeniería Electrónica
S2i 2020 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
Cofinanciación del MINECO en el marco del Programa INNPACTO (IPT-020000-2010-22)