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Descripción
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| At the Cuban Institute for Cinematographic Industry and Arts (known as ICAIC), samples were taken from several cinematographic films of cultural heritage affected by biofouling and biodeterioration problems. The films were analyzed by Environmental Electron Scanning Microscopy (ESEM) and Scanning Electron Microscopy (SEM) to determine the biodeterioration degree and the type of colonising microorganisms. To study fungal viability the films were dyed using fluorochromes and were then analysed using an epifluorescence microscope. Also, a protocol for the recovery of fungi from the surface of the films was developed. Their DNA was extracted and amplified using PCR to be automatically sequenced later, so as to identify which kinds of fungi were responsible for the biodeterioration of the cinematographic films. A significant fungal colonisation was present on both sides of the films on all the samples, whereas on some films, there was a higher concentration of fungi on one of the two sides. The degree of colonisation also varied from one film to another, and on some of them, fungi could be seen to penetrate the film itself. We have detect some of the fungi are still active and their harmful effect on the films under study can, therefore, continue. | |
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Internacional
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Si |
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Nombre congreso
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15th International Biodeterioration and Biodegradation Symposium, (IBBS-15) |
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Tipo de participación
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960 |
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Lugar del congreso
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Vienna (Austria) |
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Revisores
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Si |
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ISBN o ISSN
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DOI
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Fecha inicio congreso
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19/09/2011 |
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Fecha fin congreso
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24/09/2011 |
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