Observatorio de I+D+i UPM

Memorias de investigación
Book chapters:
Lupinus mariae-josephi, a new lupin endemic of soils with active lime and high pH in Eastern Spain, is nodulated by a new bacterial lineage within Bradyrhizobium genus In Lupin crops
Year:2011
Research Areas
  • Molecular, cellular and genetic biology
Information
Abstract
Lupinus mariae-josephi is a recently described species (Pascual 2004) able to grow in soils with high pH and active lime content in the Valencia province (Spain). L. mariae-josephi endosymbionts are extremely slow-growing bacteria with genetic and symbiotic characteristics that differentiate them from Bradyrhizobium strains nodulating Lupinus spp. native of the Iberian Peninsula and adapted to grow in acid soils. Cross-inoculation experiments revealed that all the endosymbiotic isolates from L. mariae-josephi tested are legume-host selective and are unable to nodulate species such as L. angustifolius, and L. luteus. In contrast, Bradyrhizobium strains from Lupinus spp. tested were able to nodulate L. mariae-josephi, although the nodules fixed nitrogen inefficiently. Phylogenetic analysis was performed with housekeeping genes (rrn, glnII, recA, atpD) and nodulation gene nodC. Housekeeping gene phylogeny revealed that L. mariae-josephi rhizobia form a strongly supported monophyletic group within Bradyrhizobium genus. This cluster also includes B. jicamae and certain strains of B. elkanii. Contrarily, isolates from other Lupinus spp. native of the Iberian Peninsula were grouped mainly within B. canariense and two B. japonicum lineages. Phylogenetic analysis of L. mariae-josephi isolates based on the nodC symbiotic gene defined a solid clade close to isolates from Algerian Retama spp. and to fast-growing rhizobia.
International
Si
Book Edition
Book Publishing
B.Naganowska, P. Kachlicki and B. Wolko (Eds) International Lupin Association, Canterbury, New Zealand.
ISBN
978-83-61607-73-1
Series
Book title
Proceeding of the 13th International Lupin Conference In Lupin crops ? an opportunity for today, a promise for the future
From page
243
To page
249
Participants
  • Autor: Luis Rey Navarro (UPM)
  • Autor: Carmen Sánchez Cañizares (UPM)
  • Autor: David Ricardo Duran Wendt (UPM)
  • Autor: Tomas-Andres Ruiz Argueso (UPM)
  • Autor: Juan Imperial Ródenas (UPM)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Asociaciones simbióticas planta-microorganismo
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología
S2i 2020 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
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