Observatorio de I+D+i UPM

Memorias de investigación
Research Publications in journals:
Wavelet-based image fusion in multi-view three-dimensional microscopy
Year:2012
Research Areas
  • Treatment of medical images
Information
Abstract
Motivation: Multi-view microscopy techniques such as Light-Sheet Fluorescence Microscopy (LSFM) are powerful tools for 3D + time studies of live embryos in developmental biology. The sample is imaged from several points of view, acquiring a set of 3D views that are then combined or fused in order to overcome their individual limitations. Views fusion is still an open problem despite recent contributions in the ?eld. Results: We developed a wavelet-based multi-view fusion method that, due to wavelet decomposition properties, is able to combine the complementary directional information from all available views into a single volume. Our method is demonstrated on LSFM acquisitions from live sea urchin and zebra?sh embryos. The fusion results show improved overall contrast and details when compared with any of the acquired volumes. The proposed method does not need knowledge of the system?s point spread function (PSF) and performs better than other existing PSF independent fusion methods. Availability and Implementation: The described method was implemented in Matlab (The Mathworks, Inc., USA) and a graphic user interface was developed in Java. The software, together with two sample datasets, is available at http://www.die.upm.es/im/software/SPIMFusionGUI.zip A public release, free of charge for non-commercial use, is planned after the publication of this article.
International
Si
JCR
Si
Title
Bioinformatics
ISBN
1367-4803
Impact factor JCR
5,468
Impact info
Volume
28
10.1093/bioinformatics/BTR609
Journal number
From page
238
To page
245
Month
ENERO
Ranking
Participants
  • Autor: José Luis Rubio Guivernau (UPM)
  • Autor: Vasily Gurchenkov (Institut des Systèmes complexes )
  • Autor: Miguel Angel Luengo Oroz (UPM)
  • Autor: Louise Duloquin (Institut des Systèmes complexes )
  • Autor: Paul Bourgine (Institut des Systèmes complexes )
  • Autor: Andres de Santos Lleo (UPM)
  • Autor: Nadine Peyrieras (Institut des Systèmes complexes )
  • Autor: Maria Jesus Ledesma Carbayo (UPM)
Research Group, Departaments and Institutes related
  • Creador: Departamento: Ingeniería Electrónica
S2i 2020 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
Cofinanciación del MINECO en el marco del Programa INNPACTO (IPT-020000-2010-22)