Observatorio de I+D+i UPM

Memorias de investigación
Research Publications in journals:
Methodology for Reconstructing Early Zebra?sh Development From In Vivo Multiphoton Microscopy
Year:2012
Research Areas
  • Image processing
Information
Abstract
Investigating cell dynamics during early zebra?sh embryogenesis requires speci?c image acquisition and analysis strategies. Multiharmonic microscopy, i.e., second- and third-harmonic generations, allows imaging cell divisions and cell membranes in unstained zebra?sh embryos from 1- to 1000-cell stage. This paper presents the design and implementation of a dedicated image processing pipeline (tracking and segmentation) for the reconstruction of cell dynamics during these developmental stages. This methodology allows the reconstruction of the cell lineage tree including division timings, spatial coordinates, and cell shape until the 1000-cell stage with minute temporal accuracy and micrometer spatial resolution. Data analysis of the digital embryos provides an extensive quantitative description of early zebra?sh embryogenesis.
International
Si
JCR
Si
Title
Ieee Transactions on Image Processing
ISBN
1057-7149
Impact factor JCR
2,606
Impact info
Datos JCR del año 2010
Volume
10.1109/TIP.2011.2177911
Journal number
From page
2335
To page
2340
Month
SIN MES
Ranking
Participants
  • Autor: Miguel Angel Luengo Oroz (UPM)
  • Autor: José Luis Rubio Guivernau (UPM)
  • Autor: Emmanuel Faure
  • Autor: Thierry Savy
  • Autor: Louise Duloquin
  • Autor: Nicolas Olivier
  • Autor: David Pastor Escuredo (UPM)
  • Autor: Maria Jesus Ledesma Carbayo (UPM)
  • Autor: Delphine Debarre
  • Autor: Paul Bourgine
  • Autor: Emmanuel Beaurepaire
  • Autor: Nadine Peyrieras
  • Autor: Andres de Santos Lleo (UPM)
Research Group, Departaments and Institutes related
  • Creador: Departamento: Ingeniería Electrónica
S2i 2020 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
Cofinanciación del MINECO en el marco del Programa INNPACTO (IPT-020000-2010-22)