Observatorio de I+D+i UPM

Memorias de investigación
Research Project:
Plant cIRES Biotech: Plant cIres Biotech
Year:2011
Research Areas
Information
Abstract
To cope with abiotic stresses plants require an extensive molecular regulation of gene expression. In plants, translation is a key step in the control of gene expression under abiotic stress conditions. This translational regulation involves (1) a global inhibition of protein synthesis and (2) an efficient and selective translation of certain mRNAs, generally codifying proteins involved in the abiotic stress response. Although in plants the mechanisms involved in the onset of this dual regulation are currently unknown, some evidences point out that cap independent translation, via recognition of internal ribosome entry sites (IRES) within the mRNAs efficiently translated, could be the clue for the selective protein synthesis observed under such conditions. In this proposal we aim to further characterize the cellular IRESs operating under abiotic stress conditions in plants and to exploit the identified cellular IRESs as biotechnological tools to allow the efficient and selective translation of mRNAs of interest under abiotic stress conditions. In plants, no IRES trans-acting factors (ITAFs) and only two cellular IRESs have been identified so far. Therefore, the systematic identification of new cellular IRESs, the identification for the first time of ITAFs and the study of how they can control IRES activity-specificity under abiotic stress conditions are important steps forward in the knowledge of how plants adapt to environmental stresses. In addition, the pioneering use of the identified cellular IRESs as a tool to tightly and specifically control the expression of proteins of interest under abiotic stress conditions will open up a new perspective for the study of abiotic stress in plants and for the generation of plants with increased tolerance to such conditions.
International
Si
Project type
Proyectos y convenios en convocatorias públicas competitivas
Company
Comisión Europea
Entity Nationality
BELGICA
Entity size
Grande
Granting date
24/11/2010
Participants
  • Participante: Cristina Prieto Navarro (UPM)
  • Participante: Alfonso Muñoz Gutiérrez (UPM)
  • Participante: Lourdes Fernández Calvino (UPM)
  • Participante: Marta Berrocal Lobo (UPM)
  • Participante: Nuria Fernandez Bautista-Abad (UPM)
  • Director: Fernando Garcia-Arenal Rodriguez (UPM)
  • Participante: Sira Echevarría Zomeño (UPM)
Research Group, Departaments and Institutes related
  • Creador: No seleccionado
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología
S2i 2019 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
Cofinanciación del MINECO en el marco del Programa INNPACTO (IPT-020000-2010-22)