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Descripción
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| The initial reaction in the pathway leading to the production of indole-3-acetic acid (IAA) in plants is the reaction between chorismate and glutamine to produce anthranilate, catalysed by the enzyme anthranilate synthase (ASA; EC 4.1.3.27). Compared with non-transgenic controls, leaves of transgenic poplar with ectopic expression of the pine cytosolic glutamine synthetase (GS1a; EC 6.3.1.2) produced significantly greater glutamine and significantly enhanced ASA a-subunit (ASA1) transcript and protein (approximately 130% and 120% higher than in the untransformed controls, respectively). Similarly, tobacco leaves fed with 30 mM glutamine and 2 mM chorismate showed enhanced ASA1 transcript and protein (175% and 90% higher than controls, respectively). Furthermore, free IAA was significantly elevated both in leaves of GS1a transgenic poplar and in tobacco leaves fed with 30 mM glutamine and 2 mM chorismate. These results indicated that enhanced cellular glutamine may account for the enhanced growth in GS transgenic poplars through the regulation of auxin biosynthesis | |
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Internacional
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JCR del ISI
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Título de la revista
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Journal of Experimental Botany |
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ISSN
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0022-0957 |
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Factor de impacto JCR
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4,818 |
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Información de impacto
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Volumen
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62 |
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DOI
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10.1093/jxb/err026 |
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Número de revista
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13 |
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Desde la página
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4423 |
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Hasta la página
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4431 |
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Mes
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SIN MES |
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Ranking
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