Observatorio de I+D+i UPM

Memorias de investigación
Other publications:
Registration tools to build a digital 3D cartography of early zebrafish embryogenesis
Year:2012
Research Areas
  • Electronic technology and of the communications
Information
Abstract
In order to properly model and reconstruct embryogenesis, it is crucial to provide accurate quantitative measurements, both in time and space, of a significant number of gene patterns with cellular resolution. Acquiring a sufficient amount of these gene patterns at different developmental stages requires the imaging of a large number of different individuals, each of which is stained to display only a few of these expressions. In this scenario, it becomes crucial to dispose of the necessary tools that bring together all these various acquisitions into one single digital model where all the gene expression interactions can be jointly visualized, measured and studied at once. We have developed a registration framework capable to accurately and automatically match a large number of highly variable acquisitions of zebrafish embryos into one target individual. Little human interaction is required throughout the process and a visualization software was developed that facilitates the inspection, supervision and interaction with the results. We have used these tools to generate a 3D Atlas of zebrafish gene expression which integrates information from 51 different embryos, 200.000 cells, 96 gene patterns and 6 time cohorts spanning from 3 to 6 hpf (hours post fertilization).
International
Si
Entity
IEEE Signal Processing Society
Place
Barcelona
Pages
Reference/URL
Publication type
Libro de resúmenes
Participants
  • Autor: Carlos Castro Gonzalez (UPM)
  • Autor: Miguel Angel Luengo Oroz (UPM)
  • Autor: Maria Jesus Ledesma Carbayo (UPM)
  • Autor: Andres de Santos Lleo (UPM)
Research Group, Departaments and Institutes related
  • Creador: Centro o Instituto I+D+i: Centro de tecnología Biomédica CTB
  • Departamento: Ingeniería Electrónica
S2i 2020 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
Cofinanciación del MINECO en el marco del Programa INNPACTO (IPT-020000-2010-22)