Observatorio de I+D+i UPM

Memorias de investigación
Research Publications in journals:
A cathepsin F-like peptidase involved in barley grain protein mobilization, HvPap-1, is modulated by its own propeptide and by cystatins
Year:2012
Research Areas
  • Natural sciences and health sciences,
  • Molecular biology
Information
Abstract
Among the C1A cysteine proteases, the plant cathepsin F-like group has been poorly studied. This paper describes the molecular and functional characterization of the HvPap-1 cathepsin F-like protein from barley. This peptidase is N-glycosylated and has to be processed to become active, being its own propeptide an important modulator of the peptidase activity. The expression pattern of its mRNA and protein suggest that it is involved in different proteolytic processes in the barley plant. HvPap-1 peptidase has been purified in E. coli and the recombinant protein is able to degrade different substrates, including barley grain proteins (hordeins, albumins and globulins) stored in the barley endosperm. It has been localised in protein bodies and vesicles of the embryo and it is induced in aleurones by GA treatment. These three features support the HvPap-1 implication in the storage protein mobilization during grain germination. In addition, a complex regulation exerted by the barley cystatins, which are cysteine protease inhibitors and by its own propeptide, is also described
International
Si
JCR
Si
Title
Journal of Experimental Botany
ISBN
0022-0957
Impact factor JCR
4,818
Impact info
Datos JCR del año 2010
Volume
63
10.1093/jxb/err313
Journal number
63
From page
4615
To page
4629
Month
SIN MES
Ranking
Participants
  • Autor: Ines Cambra Marin (UPM)
  • Autor: Manuel Martinez Muñoz (UPM)
  • Autor: Beatriz Dader (CSIC)
  • Autor: Pablo Gonzalez-Melendi De Leon (UPM)
  • Autor: Jacinto Gandullo (Universidad de Sevilla)
  • Autor: Estrella Santamaria (Universidad de Onatario-Canada)
  • Autor: M. Isabel Diaz Rodriguez (UPM)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Interacciones moleculares planta-insecto.
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología
S2i 2020 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
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