Observatorio de I+D+i UPM

Memorias de investigación
Research Publications in journals:
HKT2;2/1, a K+-permeable transporter identified in a salt tolerant rice cultivar through surveys of natural genetic polymorphism
Year:2012
Research Areas
  • Cell biology
Information
Abstract
We have investigated OsHKT2;1 natural variation in a collection of 49 cultivars with different levels of salt tolerance and geographical origins. The effect of identified polymorphism on OsHKT2;1 activity was analysed through heterologous expression of variants in Xenopus oocytes. OsHKT2;1 appeared to be a highly conserved protein with only five possible amino acid substitutions that have no substantial effect on functional properties. Our study, however, also identified a new HKT isoform, No-OsHKT2;2/1 in Nona Bokra, a highly salt-tolerant cultivar. No-OsHKT2;2/1 probably originated from a deletion in chromosome 6, producing a chimeric gene. Its 5¢ region corresponds to that of OsHKT2;2, whose full-length sequence is not present in Nipponbare but has been identified in Pokkali, a salt-tolerant rice cultivar. Its 3¢ region corresponds to that of OsHKT2;1. No-OsHKT2;2/1 is essentially expressed in roots and displays a significant level of expression at high Na+ concentrations, in contrast to OsHKT2;1. Expressed in Xenopus oocytes or in Saccharomyces cerevisiae, No-OsHKT2;2/1 exhibited a strong permeability to Na+ and K+, even at high external Na+ concentrations, like OsHKT2;2, and in contrast to OsHKT2;1. Our results suggest that No-OsHKT2;2/1 can contribute to Nona Bokra salt tolerance by enabling root K+ uptake under saline conditions
International
Si
JCR
Si
Title
Plant Journal
ISBN
0960-7412
Impact factor JCR
6,948
Impact info
Datos JCR del año 2010
Volume
71
Journal number
From page
750
To page
762
Month
SIN MES
Ranking
Participants
  • Autor: Ronald J.F.J. Oomen (Biochimie et physiologie Moleculaire des Plantes, Institut de Biologie Integrative des Plantes, INRA/Montpellier SupAgro/Universite Montpellier, France)
  • Autor: Begoña Benito Casado (UPM)
  • Autor: Hervé Sentenac (Biochimie et physiologie Moleculaire des Plantes, Institut de Biologie Integrative des Plantes, INRA/Montpellier SupAgro/Universite Montpellier, Franc)
  • Autor: Alonso Rodriguez Navarro (UPM)
  • Autor: Manuel Talon (Instituto Valenciano de Investigaciones Agrarias, Valencia)
  • Autor: Anne-Allienor Very (Biochimie et physiologie Moleculaire des Plantes, Institut de Biologie Integrative des Plantes, INRA/Montpellier SupAgro/Universite Montpellier, Franc)
  • Autor: Concha Domingo (Instituto Valenciano de Investigaciones Agrarias, Valencia)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Homeostasis iónica y ciclo celular
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología
S2i 2020 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
Cofinanciación del MINECO en el marco del Programa INNPACTO (IPT-020000-2010-22)