Observatorio de I+D+i UPM

Memorias de investigación
Research Publications in journals:
Expression of a barley cystatin gene in maize enhances resistance against phytophagous mites by altering their cysteine-proteases
Year:2011
Research Areas
  • Natural sciences and health sciences,
  • Molecular biology
Information
Abstract
Phytocystatins are inhibitors of cysteine-proteases from plants putatively involved in plant defence based on their capability of inhibit heterologous enzymes. We have previously characterised the whole cystatin gene family members from barley (HvCPI-1 to HvCPI-13). The aim of this study was to assess the effects of barley cystatins on two phytophagous spider mites, Tetranychus urticae and Brevipalpus chilensis. The determination of proteolytic activity profile in both mite species showed the presence of the cysteine-proteases, putative targets of cystatins, among other enzymatic activities. All barley cystatins, except HvCPI-1 and HvCPI-7, inhibited in vitro mite cathepsin L- and/or cathepsin B-like activities, HvCPI-6 being the strongest inhibitor for both mite species. Transgenic maize plants expressing HvCPI-6 protein were generated and the functional integrity of the cystatin transgene was confirmed by in vitro inhibitory effect observed against T. urticae and B. chilensis protein extracts. Feeding experiments impaired on transgenic lines performed with T. urticae impaired mite development and reproductive performance. Besides, a significant reduction of cathepsin L-like and/or cathepsin B-like activities was observed when the spider mite fed on maize plants expressing HvCPI-6 cystatin. These findings reveal the potential of barley cystatins as acaricide proteins to protect plants against two important mite pests.
International
Si
JCR
Si
Title
Plant Cell Reports
ISBN
0721-7714
Impact factor JCR
2,279
Impact info
Datos JCR del año 2010
Volume
30
10.1007/s00299-010-0948-z
Journal number
From page
101
To page
112
Month
SIN MES
Ranking
Participants
  • Autor: Laura Carrillo
  • Autor: Manuel Martinez Muñoz (UPM)
  • Autor: Koreen Ramessar
  • Autor: Ines Cambra Marin (UPM)
  • Autor: Pedro Castañera
  • Autor: Felix Ortego
  • Autor: M. Isabel Diaz Rodriguez (UPM)
Research Group, Departaments and Institutes related
  • Creador: Departamento: Biotecnología
S2i 2020 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
Cofinanciación del MINECO en el marco del Programa INNPACTO (IPT-020000-2010-22)