Observatorio de I+D+i UPM

Memorias de investigación
Research Publications in journals:
Monte-Carlo Dosimetry on a Realistic Cell Monolayer Geometry Exposed to Alpha-Particles
Year:2012
Research Areas
  • Physics - Structure of materials,
  • Physics - Radiation physics
Information
Abstract
The energy and specific energy absorbed in the main cell compartments (nucleus and cytoplasm) in typical radiobiology experiments are usually estimated by calculations as they are not accessible for a direct measurement. In most of the work, the cell geometry is modelled using the combination of simple mathematical volumes. We propose a method based on high resolution confocal imaging and ion beam analysis (IBA) in order to import realistic cell nuclei geometries in Monte-Carlo simulations and thus take into account the variety of different geometries encountered in a typical cell population. Seventy-six cell nuclei have been imaged using confocal microscopy and their chemical composition has been measured using IBA. A cellular phantom was created from these data using the ImageJ image analysis software and imported in the Geant4 Monte-Carlo simulation toolkit. Total energy and specific energy distributions in the 76 cell nuclei have been calculated for two types of irradiation protocols: a 3 MeV alpha particle microbeam used for targeted irradiation and a 239Pu alpha source used for large angle random irradiation. Qualitative images of the energy deposited along the particle tracks have been produced and show good agreement with images of DNA double strand break signalling proteins obtained experimentally. The methodology presented in this paper provides microdosimetric quantities calculated from realistic cellular volumes. It is based on open-source oriented software that is publicly available.
International
Si
JCR
Si
Title
PHYSICS IN MEDICINE AND BIOLOGY
ISBN
0031-9155
Impact factor JCR
2,829
Impact info
Volume
57
doi:10.1088/0031-9155/57/8/2189
Journal number
From page
2189
To page
2207
Month
MARZO
Ranking
Q1
Participants
  • Autor: Ph. Barberet
  • Autor: F. Vianna
  • Autor: M. Karamitros
  • Autor: T. Brun
  • Autor: Nuria Gordillo Garcia (UPM)
  • Autor: Ph. Moretto
  • Autor: S. Incerti
  • Autor: H. Seznec
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Fusión Nuclear Inercial y Tecnología de fusión
  • Departamento: Ingeniería Nuclear
S2i 2020 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
Cofinanciación del MINECO en el marco del Programa INNPACTO (IPT-020000-2010-22)