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Descripción
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| Deciphering the molecular basis of toxicology mechanism induced by nanoparticles (NPs) remains an essential challenge. Ion Beam Analysis (IBA) was applied in combination with Transmission Electron Microscopy and Confocal Microscopy to analyze human cells and Caenorhabditis elegans (C. elegans) exposed to TiO2-NPs. Investigating chemical elemental distributions using IBA gives rise to a fine quantification of the TiO2-NPs uptake within a cell and to the determination of the intracellular chemical modifications after TiO2-NPs internalization. In addition, fluorescent dye-modified TiO2-NPs have been synthesized to allow their detection, precise quantification and tracking in vitro. We demonstrated that internalization of these TiO2-NPs altered the calcium homeostasis and induced a decrease in cell proliferation associated with an early cell differentiation, without any indication of cell death. Finally, the relation between the surface chemistry of the TiO2-NPs and their in vitro toxicity is clearly established and emphasizes the importance of the calcium homeostasis alteration in response to the presence of TiO2-NPs [1]. Additionally, we will also present our last developments and data obtained using IBA and IBA-tomography on C. elegans. [1] Simon et al. Nanotoxicology, June 2011; 5(2): 125?139 | |
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Internacional
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Si |
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Nombre congreso
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13th INTERNATIONAL CONFERENCE ON NUCLEAR MICROPROBE TECHNOLOGY & APPLICATIONS 2012 |
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Tipo de participación
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960 |
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Lugar del congreso
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Lisbon (Portugal) |
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Revisores
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Si |
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ISBN o ISSN
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DOI
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Fecha inicio congreso
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22/07/2012 |
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Fecha fin congreso
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27/07/2012 |
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Título de las actas
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