Observatorio de I+D+i UPM

Memorias de investigación
Research Publications in journals:
Calcium-dependent modulation and plasma membrane targeting of the AKT2 potassium channel by the CBL4/CIPK6 calcium sensor/protein kinase complex.
Year:2011
Research Areas
  • Plants and animal biology and ecology
Information
Abstract
Potassium (K(+)) channel function is fundamental to many physiological processes. However, components and mechanisms regulating the activity of plant K(+) channels remain poorly understood. Here, we show that the calcium (Ca(2+)) sensor CBL4 together with the interacting protein kinase CIPK6 modulates the activity and plasma membrane (PM) targeting of the K(+) channel AKT2 from Arabidopsis thaliana by mediating translocation of AKT2 to the PM in plant cells and enhancing AKT2 activity in oocytes. Accordingly, akt2, cbl4 and cipk6 mutants share similar developmental and delayed flowering phenotypes. Moreover, the isolated regulatory C-terminal domain of CIPK6 is sufficient for mediating CBL4- and Ca(2+)-dependent channel translocation from the endoplasmic reticulum membrane to the PM by a novel targeting pathway that is dependent on dual lipid modifications of CBL4 by myristoylation and palmitoylation. Thus, we describe a critical mechanism of ion-channel regulation where a Ca(2+) sensor modulates K(+) channel activity by promoting a kinase interaction-dependent but phosphorylation-independent translocation of the channel to the PM.
International
Si
JCR
Si
Title
Cell Research
ISBN
1001-0602
Impact factor JCR
9,417
Impact info
Datos JCR del ańo 2010 times cited: 4 (05.12.2012)
Volume
21
Journal number
From page
1116
To page
1130
Month
JULIO
Ranking
23/181 CELL BIOLOGY (SCI)
Participants
  • Autor: Katrin Held (Universität Münster, Germany)
  • Autor: François Pascaud (INRA Montpellier, France)
  • Autor: Christian Eckert (Universität Münster, Germany)
  • Autor: Pawel Gajdanowicz (Universität Potsdam, Germany)
  • Autor: Kenji Hashimoto (Universität Münster, Germany)
  • Autor: Claire Corratgé-Faillie (INRA Montpellier, France)
  • Autor: Jan Niklas Offenborn (Universität Münster, Germany)
  • Autor: Benoît Lacombe (INRA Montpellier, France)
  • Autor: Ingo Dreyer . (UPM)
  • Autor: Jean-Baptiste Thibaud (INRA Montpellier, France)
  • Autor: Jörg Kudla (Universität Münster, Germany)
Research Group, Departaments and Institutes related
  • Creador: Departamento: Biotecnología
S2i 2020 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
Cofinanciación del MINECO en el marco del Programa INNPACTO (IPT-020000-2010-22)