Observatorio de I+D+i UPM

Memorias de investigación
Research Publications in journals:
The putative K+ channe subunit AtKCO3 forms stable dimers in Arabidopsis.
Year:2012
Research Areas
  • Plants and animal biology and ecology
Information
Abstract
The permeation pore of K(+) channels is formed by four copies of the pore domain. AtKCO3 is the only putative voltage-independent K(+) channel subunit of Arabidopsis thaliana with a single pore domain. KCO3-like proteins recently emerged in evolution and, to date, have been found only in the genus Arabidopsis (A. thaliana and A. lyrata). We show that the absence of KCO3 does not cause marked changes in growth under various conditions. Only under osmotic stress we observed reduced root growth of the kco3-1 null-allele line. This phenotype was complemented by expressing a KCO3 mutant with an inactive pore, indicating that the function of KCO3 under osmotic stress does not depend on its direct ability to transport ions. Constitutively overexpressed AtKCO3 or AtKCO3::GFP are efficiently sorted to the tonoplast indicating that the protein is approved by the endoplasmic reticulum quality control. However, vacuoles isolated from transgenic plants do not have significant alterations in current density. Consistently, both AtKCO3 and AtKCO3::GFP are detected as homodimers upon velocity gradient centrifugation, an assembly state that would not allow for activity. We conclude that if AtKCO3 ever functions as a K(+) channel, active tetramers are held by particularly weak interactions, are formed only in unknown specific conditions and may require partner proteins.
International
Si
JCR
No
Title
Frontiers in Plant Science
ISBN
1664-462X
Impact factor JCR
Impact info
Volume
3
Journal number
251
From page
1
To page
13
Month
NOVIEMBRE
Ranking
Participants
  • Autor: Alessandra Rocchetti (Istituto di Biologia e Biotecnologia Agraria, CNR Milano, Italy)
  • Autor: Tripti Sharma (Universität Potsdam, Germany)
  • Autor: Camilla Wulfetange (Universität Potsdam, Germany)
  • Autor: Joachim Scholz-Starke (Istituto di Biofisica, CNR Genova, Italy)
  • Autor: Alexandra Grippa (Istituto di Biologia e Biotecnologia Agraria, CNR Milano, Italy)
  • Autor: Armando Carpaneto (Istituto di Biofisica, CNR Genova, Italy)
  • Autor: Ingo Dreyer . (UPM)
  • Autor: Alessandro Vitale (Istituto di Biologia e Biotecnologia Agraria, CNR Milano, Italy)
  • Autor: Katrin Czempinski (Universität Potsdam, Germany)
  • Autor: Emanuela Pedrazzini (Istituto di Biologia e Biotecnologia Agraria, CNR Milano, Italy)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Redes Reguladoras del Desarrollo y Respuesta a Estrés en Plantas
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología
S2i 2020 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
Cofinanciación del MINECO en el marco del Programa INNPACTO (IPT-020000-2010-22)