Observatorio de I+D+i UPM

Memorias de investigación
Research Publications in journals:
Molecular evolution of slow and quick anion channels (SLACs and QUACs/ALMTs).
Year:2012
Research Areas
  • Plants and animal biology and ecology
Information
Abstract
Electrophysiological analyses conducted about 25 years ago detected two types of anion channels in the plasma membrane of guard cells. One type of channel responds slowly to changes in membrane voltage while the other responds quickly. Consequently, they were named SLAC, for SLow Anion Channel, and QUAC, for QUick Anion Channel. Recently, genes SLAC1 and QUAC1/ALMT12, underlying the two different anion current components, could be identified in the model plant Arabidopsis thaliana. Expression of the gene products in Xenopus oocytes confirmed the quick and slowcurrent kinetics. In this study we provide an overview on our current knowledge on slow and quick anion channels in plants and analyze the molecular evolution of ALMT/QUAC-like and SLAC-like channels. We discovered fingerprints that allow screening databases for these channel types and were able to identify 192 (177 non-redundant) SLAC-like and 422 (402 non-redundant) ALMT/QUAC-like proteins in the fully sequenced genomes of 32 plant species. Phylogenetic analyses provided new insights into the molecular evolution of these channel types. We also combined sequence alignment and clustering with predictions of protein features, leading to the identification of known conserved phosphorylation sites in SLAC1-like channels along with potential sites that have not been yet experimentally confirmed. Using a similar strategy to analyze the hydropathicity of ALMT/QUAC-like channels, we propose a modified topology with additional transmembrane regions that integrates structure and function of these membrane proteins. Our results suggest that cross-referencing phylogenetic analyses with position-specific protein properties and functional data could be a very powerful tool for genome research approaches in general.
International
Si
JCR
No
Title
Frontiers in Plant Science
ISBN
1664-462X
Impact factor JCR
Impact info
Volume
3
10.3389/fpls.2012.00263
Journal number
263
From page
1
To page
12
Month
NOVIEMBRE
Ranking
Participants
  • Autor: Ingo Dreyer . (UPM)
  • Autor: Judith Lucia Gomez Porras (UPM)
  • Autor: Diego Mauricio Riaño-Pachón (Universidad de los Andes, Bogotá, Colombia)
  • Autor: Rainer Hedrich (Universität Würzburg, Germany)
  • Autor: Dietmar Geiger (Universität Würzburg, Germany)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Biotecnología de Proteínas Vegetales
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Grupo de Investigación: Redes Reguladoras del Desarrollo y Respuesta a Estrés en Plantas
  • Departamento: Biotecnología
S2i 2020 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
Cofinanciación del MINECO en el marco del Programa INNPACTO (IPT-020000-2010-22)