Memorias de investigación
Artículos en revistas:
Isozyme systems as markers of genetic deterioration in stored seeds of Brassicaceae species
Año:2012
Áreas de investigación
  • Ciencias naturales y ciencias de la salud
Datos
Descripción
The main objective of this study was to determine if isozyme systems can be used as markers of genetic deterioration in Brassicaceae seed accessions under different storage conditions. Seed samples of Brassica oleracea, Cardaria draba, Erysimum cheiri, Iberis sempervirens and Rapistrum rugosum were stored for periods of 9 to 30 years at -10°C and 3-4% seed moisture content (long-term or LT conditions) and at 5°C and uncontrolled relative humidity (RH) (short-term or ST conditions). Starch Gel Electrophoresis (SGE) was used to analyse six enzyme systems oriented to determine the genetic deterioration of the accessions studied. The results obtained show that long-term storage conditions (LT) were extremely effective in maintaining the viability of seeds of the five Brassicaceae species studied. The final germination percentages reached by seeds from LT samples ranged from 75 to 100%, while the germination percentages of ST samples (except for B. oleracea) were very low (from 0 to 10%). Similar conclusions were obtained studying the integrity of electrophoretic bands for several isozymes. Two enzyme systems were of special interest: malate dehydrogenase and alcohol dehydrogenase.
Internacional
Si
JCR del ISI
Si
Título de la revista
Seed Science And Technology
ISSN
0251-0952
Factor de impacto JCR
0,62
Información de impacto
Datos JCR del año 2011
Volumen
DOI
Número de revista
40
Desde la página
365
Hasta la página
373
Mes
SIN MES
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Participantes
  • Autor: M. Itziar Josefina Aguinagalde Madariaga UPM
  • Autor: Silvia Maselli Departamento de Biología vegetal, Universidad Politécnica de Madrid
  • Autor: Felix Perez Garcia UPM
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Grupo de Investigación: Biodiversidad y conservación de recursos fitogenéticos
  • Departamento: Biología Vegetal