Observatorio de I+D+i UPM

Memorias de investigación
Research Publications in journals:
Annotated genetic linkage maps of Pinus pinaster Ait. from a Central Spain population using microsatellite and gene based markers
Year:2012
Research Areas
  • Natural sciences and health sciences
Information
Abstract
Abstract Background: Pinus pinaster Ait. is a major resin producing species in Spain. Genetic linkage mapping can facilitate marker-assisted selection (MAS) through the identification of Quantitative Trait Loci and selection of allelic variants of interest in breeding populations. In this study, we report annotated genetic linkage maps for two individuals (C14 and C15) belonging to a breeding program aiming to increase resin production. We use different types of DNA markers, including last-generation molecular markers. Results: We obtained 13 and 14 linkage groups for C14 and C15 maps, respectively. A total of 211 and 215 markers were positioned on each map and estimated genome length was between 1,870 and 2,166 cM respectively, which represents near 65% of genome coverage. Comparative mapping with previously developed genetic linkage maps for P. pinaster based on about 60 common markers enabled aligning linkage groups to this reference map. The comparison of our annotated linkage maps and linkage maps reporting QTL information revealed 11 annotated SNPs in candidate genes that co-localized with previously reported QTLs for wood properties and water use efficiency. Conclusions: This study provides genetic linkage maps from a Spanish population that shows high levels of genetic divergence with French populations from which segregating progenies have been previously mapped. These genetic maps will be of interest to construct a reliable consensus linkage map for the species. The importance of developing functional genetic linkage maps is highlighted, especially when working with breeding populations for its future application in MAS for traits of interest.
International
Si
JCR
Si
Title
Bmc Genomics
ISBN
1471-2164
Impact factor JCR
4,073
Impact info
Volume
13
Journal number
From page
527
To page
527
Month
SIN MES
Ranking
Biotechnology and applied microbiology 26/158 (2011) Genetics and heredity 34/158
Participants
  • Autor: M de Miguel (INIA)
  • Autor: N de María (INIA)
  • Autor: MA Guevara (INIA)
  • Autor: L Díaz (INIA)
  • Autor: E Sáez-Laguna (INIA)
  • Autor: D Sánchez-Gómez (INIA)
  • Autor: E Chancerel (INRA)
  • Autor: I Aranda (INIA)
  • Autor: Maria Carmen Collada Collada (UPM)
  • Autor: C Plomion (INRA)
  • Autor: JA Cabezas (INIA)
  • Autor: MT Cervera
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Genética y Fisiología Forestal
  • Departamento: Biotecnología
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