Observatorio de I+D+i UPM

Memorias de investigación
Communications at congresses:
Arabidopsis thaliana and its natural endophytes: a model system for the study of the ecological and molecular factors involved in endophytic interactions
Research Areas
  • Crop production
Arabidopsis thaliana L. (Brassicaceae) is the laboratory plant model par excellence. The genome of several genotypes has been sequenced and there is a large amount of available information about its functional genetics and genomics and a variety of tools to deepen in the knowledge of plant biology. For over 20 years, A. thaliana has been developed as the model organism for molecular plant genetics, including the analysis of the mechanisms of resistance to parasites. However, A. thaliana is not just a lab model plant: it is an annual species with a worldwide distribution which can be found in a variety of anthropic and wild habitats. The knowledge generated in the lab is being applied to the study of its wild populations, so it has become a model, as well, for the study of plant ecology, adaptation and evolution. More recently, the ecological and evolutive aspects of plant-microbe interactions are also being studied using. However, the study of interactions other than highly pathogenic with A. thaliana is now just sprouting, with only few published examples. We have surveyed different populations of A. thaliana from Central Spain, which is considered an origin and diversification centre of the species,. The prospected A. thaliana populations are situated at different ecological environments at the Central Plateau of Spain, and have been very well characterized for their genetic variation and geographic structure. We have isolated a high diverse number of fungal species from above ground organs of asymptomatic plants. OTUs abundance distribution shows the typical pattern of microbial communities, with a long right tail: 59% of the species are represented by just one isolate and just eight species account for 50% of the isolates. The species accumulation curve is non-asymptotic, so the sampling effort has been insufficient to approach the total number of different OTUs. These preliminary results indicate that the diversity, structure and composition of the fungal endophytic assemblages of natural occurring A. thaliana. populations is similar to those found in surveys of other hosts in temperate regions. Isolates of the most abundant genera were chosen in order to set into a point their inoculation to A. thaliana plants under controlled conditions and investigate the outcome of the interactions. This is, to our knowledge, the first work that investigates the endophytic mycobiota of A. thaliana, and one of the few in which the outcome of the interactions has been assayed under controlled conditions, validating the description of the isolates as endophytes. The availability of endophytic fungal isolates naturally infecting A. thaliana offers a great opportunity for the molecular approach of the factors affecting the interactions between endophytes and plants.
Current aspects of European endophyte research. COST Action FA1103
Reims (Francia)
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COST: Current aspects of European endophyte research
  • Autor: E. García
  • Autor: Angela Alonso Gonzalez (UPM)
  • Autor: G. Platas
  • Autor: Soledad Sacristan Benayas (UPM)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Patología Vegetal
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología
S2i 2020 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
Cofinanciación del MINECO en el marco del Programa INNPACTO (IPT-020000-2010-22)