Observatorio de I+D+i UPM

Memorias de investigación
Research Publications in journals:
Deep sequencing reveals persistence of intra- and inter-host genetic diversity in natural and greenhouse populations of zucchini yellow mosaic virus
Year:2012
Research Areas
  • Viral pathogens of plants,
  • Crop production
Information
Abstract
The genetic diversity present in populations of RNA viruses is likely to be strongly modulated by aspects of their life history, including mode of transmission. However, how transmission mode shapes patterns of intra- and inter-host genetic diversity, particularly when acting in combination with de novo mutation, population bottlenecks and the selection of advantageous mutations, is poorly understood. To address these issues, this study performed ultradeep sequencing of zucchini yellow mosaic virus in a wild gourd, Cucurbita pepo ssp. texana, under two infection conditions: aphid vectored and mechanically inoculated, achieving a mean coverage of approximately 10 000!. It was shown that mutations persisted during inter-host transmission events in both the aphid vectored and mechanically inoculated populations, suggesting that the vector-imposed transmission bottleneck is not as extreme as previously supposed. Similarly, mutations were found to persist within individual hosts, arguing against strong systemic bottlenecks. Strikingly, mutations were seen to go to fixation in the aphid-vectored plants, suggestive of a major fitness advantage, but remained at low frequency in the mechanically inoculated plants. Overall, this study highlights the utility of ultradeep sequencing in providing high-resolution data capable of revealing the nature of virus evolution, particularly as the full spectrum of genetic diversity within a population may not be uncovered without sequence coverage of at least 2500-fold.
International
Si
JCR
Si
Title
Journal of General Virology
ISBN
0022-1317
Impact factor JCR
3,363
Impact info
Volume
93
10.1099/vir.0.042622-0
Journal number
From page
1831
To page
1840
Month
SIN MES
Ranking
Participants
  • Autor: H. E. Simmons
  • Autor: J. P. Dunham
  • Autor: J. C. Stack
  • Autor: B. J. A. Dickins
  • Autor: Jesus Israel Pagan Muñoz (UPM)
  • Autor: E. C. Holmes
  • Autor: A. G. Stephenson
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Patología Vegetal
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
S2i 2020 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
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