Memorias de investigación
Artículos en revistas:
The involvement of thaumatin-like proteins in plant food cross-reactivity: a multicenter study using a specific protein microarray.
Año:2012
Áreas de investigación
  • Biología molecular, celular y genética
Datos
Descripción
Cross-reactivity of plant foods is an important phenomenon in allergy, with geographical variations with respect to the number and prevalence of the allergens involved in this process, whose complexity requires detailed studies. We have addressed the role of thaumatin-like proteins (TLPs) in cross-reactivity between fruit and pollen allergies. A representative panel of 16 purified TLPs was printed onto an allergen microarray. The proteins selected belonged to the sources most frequently associated with peach allergy in representative regions of Spain. Sera from two groups of well characterized patients, one with allergy to Rosaceae fruit (FAG) and another against pollens but tolerant to food-plant allergens (PAG), were obtained from seven geographical areas with different environmental pollen profiles. Cross-reactivity between members of this family was demonstrated by inhibition assays. Only 6 out of 16 purified TLPs showed noticeable allergenic activity in the studied populations. Pru p 2.0201, the peach TLP (41%), chestnut TLP (24%) and plane pollen TLP (22%) proved to be allergens of probable relevance to fruit allergy, being mainly associated with pollen sensitization, and strongly linked to specific geographical areas such as Barcelona, Bilbao, the Canary Islands and Madrid. The patients exhibited >50% positive response to Pru p 2.0201 and to chestnut TLP in these specific areas. Therefore, their recognition patterns were associated with the geographical area, suggesting a role for pollen in the sensitization of these allergens. Finally, the co-sensitizations of patients considering pairs of TLP allergens were analyzed by using the co-sensitization graph associated with an allergen microarray immunoassay. Our data indicate that TLPs are significant allergens in plant food allergy and should be considered when diagnosing and treating pollen-food allergy.
Internacional
Si
JCR del ISI
Si
Título de la revista
Plos One
ISSN
1932-6203
Factor de impacto JCR
4,092
Información de impacto
Volumen
7
DOI
10.1371/journal.pone.0044088
Número de revista
9
Desde la página
1
Hasta la página
11
Mes
SIN MES
Ranking
Esta actividad pertenece a memorias de investigación
Participantes
  • Autor: Aranzazu Palacín Gómez UPM
  • Autor: Luis A Rivas INTA-CSIC
  • Autor: Cristina Gomez Casado UPM
  • Autor: Jacobo Aguirre INTA-CSIC
  • Autor: Leticia Tordesillas Villuendas UPM
  • Autor: Joan Bartra CIBERES
  • Autor: Carlos Blanco Instituto de Investigacio¿n Sanitaria Princesa
  • Autor: Teresa Carrillo Hospital Universitario de Gran Canaria Dr. Negr¿n,
  • Autor: Javier Cuesta-Herranz Fundacion Jimenez D¿az
  • Autor: Jose A Cumplido Bonny Hospital Universitario de Gran Canaria Dr. Negr¿n
  • Autor: Enrique Flores Hospital General Universitario, Alicante
  • Autor: Mar G Garc¿¿a-Alvarez-Eire Complexo Hospitalario, Ourense
  • Autor: Ignacio Garc¿a-Nuñez Fundacio¿n IMABIS-Carlos Haya Hospital
  • Autor: Francisco J Fernandez Hospital General Universitario, Alicante
  • Autor: Pedro Gamboa Hospital de Basurto
  • Autor: Rosa Muñoz CIBERES
  • Autor: Maria Rosa Sanchez-Monge Y Laguna de Rins UPM
  • Autor: Maria Torres Universidad Complutense
  • Autor: Susana Varela Losada Complexo Hospitalario, Ourense
  • Autor: Mayte Villalba Universidad Complutense
  • Autor: FRancisco Vega Instituto de Investigacio¿n Sanitaria Princesa
  • Autor: Victor Parro INTA-CSIC
  • Autor: Miguel Blanca Fundacio¿n IMABIS-Carlos Haya Hospital
  • Autor: Gabriel Salcedo UPM
  • Autor: Araceli Diaz Perales UPM
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Grupo de Investigación: Biotecnología de Proteínas Vegetales
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología