Observatorio de I+D+i UPM

Memorias de investigación
Research Publications in journals:
The involvement of thaumatin-like proteins in plant food cross-reactivity: a multicenter study using a specific protein microarray.
Research Areas
  • Molecular, cellular and genetic biology
Cross-reactivity of plant foods is an important phenomenon in allergy, with geographical variations with respect to the number and prevalence of the allergens involved in this process, whose complexity requires detailed studies. We have addressed the role of thaumatin-like proteins (TLPs) in cross-reactivity between fruit and pollen allergies. A representative panel of 16 purified TLPs was printed onto an allergen microarray. The proteins selected belonged to the sources most frequently associated with peach allergy in representative regions of Spain. Sera from two groups of well characterized patients, one with allergy to Rosaceae fruit (FAG) and another against pollens but tolerant to food-plant allergens (PAG), were obtained from seven geographical areas with different environmental pollen profiles. Cross-reactivity between members of this family was demonstrated by inhibition assays. Only 6 out of 16 purified TLPs showed noticeable allergenic activity in the studied populations. Pru p 2.0201, the peach TLP (41%), chestnut TLP (24%) and plane pollen TLP (22%) proved to be allergens of probable relevance to fruit allergy, being mainly associated with pollen sensitization, and strongly linked to specific geographical areas such as Barcelona, Bilbao, the Canary Islands and Madrid. The patients exhibited >50% positive response to Pru p 2.0201 and to chestnut TLP in these specific areas. Therefore, their recognition patterns were associated with the geographical area, suggesting a role for pollen in the sensitization of these allergens. Finally, the co-sensitizations of patients considering pairs of TLP allergens were analyzed by using the co-sensitization graph associated with an allergen microarray immunoassay. Our data indicate that TLPs are significant allergens in plant food allergy and should be considered when diagnosing and treating pollen-food allergy.
Plos One
Impact factor JCR
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  • Autor: Aranzazu Palacín Gómez (UPM)
  • Autor: Luis A Rivas (INTA-CSIC)
  • Autor: Cristina Gomez Casado (UPM)
  • Autor: Jacobo Aguirre (INTA-CSIC)
  • Autor: Leticia Tordesillas Villuendas (UPM)
  • Autor: Joan Bartra (CIBERES)
  • Autor: Carlos Blanco (Instituto de Investigacio¿n Sanitaria Princesa)
  • Autor: Teresa Carrillo (Hospital Universitario de Gran Canaria Dr. Negr¿n,)
  • Autor: Javier Cuesta-Herranz (Fundacion Jimenez D¿az)
  • Autor: Jose A Cumplido Bonny (Hospital Universitario de Gran Canaria Dr. Negr¿n)
  • Autor: Enrique Flores (Hospital General Universitario, Alicante)
  • Autor: Mar G Garc¿¿a-Alvarez-Eire (Complexo Hospitalario, Ourense)
  • Autor: Ignacio Garc¿a-Nuñez (Fundacio¿n IMABIS-Carlos Haya Hospital)
  • Autor: Francisco J Fernandez (Hospital General Universitario, Alicante)
  • Autor: Pedro Gamboa (Hospital de Basurto)
  • Autor: Rosa Muñoz (CIBERES)
  • Autor: Maria Rosa Sanchez-Monge Y Laguna de Rins (UPM)
  • Autor: Maria Torres (Universidad Complutense)
  • Autor: Susana Varela Losada (Complexo Hospitalario, Ourense)
  • Autor: Mayte Villalba (Universidad Complutense)
  • Autor: FRancisco Vega (Instituto de Investigacio¿n Sanitaria Princesa)
  • Autor: Victor Parro (INTA-CSIC)
  • Autor: Miguel Blanca (Fundacio¿n IMABIS-Carlos Haya Hospital)
  • Autor: Gabriel Salcedo (UPM)
  • Autor: Araceli Diaz Perales (UPM)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Biotecnología de Proteínas Vegetales
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología
S2i 2020 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
Cofinanciación del MINECO en el marco del Programa INNPACTO (IPT-020000-2010-22)