Observatorio de I+D+i UPM

Memorias de investigación
Research Publications in journals:
Effect of Pru p 3 on dendritic cell maturation and T-lymphocyte proliferation in peach allergic patients.
Year:2012
Research Areas
  • Molecular, cellular and genetic biology
Information
Abstract
BACKGROUND: Pru p 3 is the major peach allergen and the most frequent cause of food allergy in adults in the Mediterranean area. Although its allergenicity is well characterized, its ability to generate a T-cell response is not completely known. OBJECTIVE: To investigate the influence of Pru p 3 allergen on dendritic cell (DC) maturation and specific T-cell response (T(H)1/T(H)2) in peach allergic patients. METHODS: Peach allergic patients (n = 11) and tolerant controls (n = 14) were included in the study. Monocyte-derived DC maturation after incubation with Pru p 3 was evaluated by the increase of maturational markers (CD80, CD86, and CD83) by flow cytometry. Lymphocyte proliferation was evaluated by coculturing monocyte-derived DCs and 5,6-carboxyfluorescein diacetate N-succinimidyl ester-stained lymphocytes with different concentrations of Pru p 3 (25, 10, and 1 ?g/mL) by flow cytometry and cytokine production. RESULTS: Pru p 3 induced a significant increase in the CD80, CD86, and CD83 expression on stimulated DCs from patients compared with controls. The lymphocyte proliferative response after Pru p 3 stimulation was also significantly higher along with an increase in interleukin 8 in patients compared with tolerant controls. CONCLUSION: Pru p 3 allergen induces changes in DC maturational status mainly in peach allergic patients. An increase in lymphocyte proliferative response accompanied with a different cytokine pattern was also observed compared with healthy controls.
International
Si
JCR
Si
Title
Annals of Allergy Asthma & Immunology
ISBN
1081-1206
Impact factor JCR
2,833
Impact info
Volume
109
10.1016/j.anai.2012.05.017
Journal number
1
From page
52
To page
58
Month
JULIO
Ranking
Participants
  • Autor: Enrique Gomez (Carlos Haya Hospital,)
  • Autor: Araceli Diaz Perales (UPM)
  • Autor: Leticia Tordesillas Villuendas (UPM)
  • Autor: Inmaculada Doña (Carlos Haya Hospital)
  • Autor: MAria J Torres (Carlos Haya Hospital)
  • Autor: Ana B Blazquez (Carlos Haya Hospital)
  • Autor: Francisca Gomez (Carlos Haya Hospital)
  • Autor: Miguel Blanca (Carlos Haya Hospital)
  • Autor: Cristobalina Mayorga (Carlos Haya Hospital)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Biotecnología de Proteínas Vegetales
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología
S2i 2020 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
Cofinanciación del MINECO en el marco del Programa INNPACTO (IPT-020000-2010-22)