Observatorio de I+D+i UPM

Memorias de investigación
Communications at congresses:
IDENTIFICATION OF A TYPE III SECRETION SYSTEM IN BRADYRHIZOBIUM SP.LMJC (LUPINUS)
Year:2012
Research Areas
  • Molecular, cellular and genetic biology
Information
Abstract
Lupinus mariae-josephae is a unique lupine confined to basic soils (terra rossa) of a small area in Eastern Spain. Analysis of the genome sequence of Bradyrhizobium sp. LmjC, a strain isolated from root nodules of L. mariae-josephae revealed the presence of a type III secretion system (T3SS) in a cluster of 30 genes. The genetic organization, possible gene functions and comparison with other T3SS were studied. Genes identified in this cluster include those encoding for the transcriptional activator TtsI, core components of the secretion apparatus, secreted proteins, as well as hypothetical and unknown proteins. The highest conservation was observed with T3SS structural components of B. japonicum USDA110, B. elkanii USDA61 and Mesorhizobium loti MAF303099. One of the genes in the LmjC T3SS cluster encodes for a protein homologous to NopE1 and NopE2 proteins secreted by the T3SS of B. japonicum. NopEs from B. japonicum contain two DUF1521 domains of unknown function, but are involved in self-cleavage, and are present in a few uncharacterized proteins of plant-associated bacteria (1). The NopE-like protein from LmjC presents only one DUF1521 motif. Consistent with this, the purified protein (after its expression in E. coli) exhibited one cleavage site in the presence of calcium, unlike the two cleavage sites observed in NopEs from B. japonicum. Self-cleavage of LmjC NopE also happened in the presence of Cu, Cd, Zn and Mn, but not in presence of Ni, Co or Mg. Site-directed mutagenesis of the DUF1521 motif in LmjC NopE abolished in vitro self-cleavage.
International
Si
Congress
10th European Nitrogen Fixation Conference
960
Place
Munich, Alemania
Reviewers
Si
ISBN/ISSN
000-000-000-0
Start Date
02/09/2012
End Date
05/09/2012
From page
610
To page
610
Book of abstract
Participants
  • Autor: David Ricardo Duran Wendt (UPM)
  • Autor: Tomas-Andres Ruiz Argueso (UPM)
  • Autor: Juan Imperial Ródenas (UPM)
  • Autor: Victor Pastor (UPM)
  • Autor: Luis Rey Navarro (UPM)
  • Autor: Pablo Lopez (UPM)
  • Autor: Susanne Zehner (Universidad Tecnica de Dresde)
  • Autor: Michael Göttfert (Universidad Tecnica de Dresde)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Asociaciones simbióticas planta-microorganismo
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología
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