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Descripción
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| Analysis of Rhizobium leguminosarum bv. viciae strain 3841 genome sequence revealed a genetic system (RL1175 through RL1178), potentially involved in nickel homeostasis. The predicted RL1175 gene product shows sequence similarity to cation diffusion facilitator proteins, whereas RL1178 encodes a high-affinity nickel uptake transporter of the NiCoT family (nickel-cobalt transport) and the RL1176 gene product is an NreA-like nickel- responsive regulator. The confluence of uptake and efflux transport proteins potentially linked in the same genetic system resembles the organization of the ncrABC and nreBAC operons from Serratia marcenses and Legionella pneumophila, involved in nickel and cobalt resistance. Gene expression analysis using promoter fusions to lacZ and qRT-PCR assays revealed that the identified R. leguminosarum genes are organized as a single operon whose transcription is strongly induced by nickel, but not cobalt, ions in free-living cultures, as well as in pea bacteroid cells. Generation of mutants affected in this system is in progress and will hopefully help to define the role of the interplay between metal uptake and metal efflux systems in nickel resistance. | |
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Internacional
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Si |
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Nombre congreso
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10th European Nitrogen Fixation Conference |
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Tipo de participación
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960 |
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Lugar del congreso
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Munich, Alemania |
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Revisores
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Si |
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ISBN o ISSN
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00000-00000 |
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DOI
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Fecha inicio congreso
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02/09/2012 |
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Fecha fin congreso
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05/09/2012 |
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Desde la página
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23 |
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Hasta la página
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23 |
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Título de las actas
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Abstract book |