Observatorio de I+D+i UPM

Memorias de investigación
Communications at congresses:
IDENTIFICATION AND ANALYSIS OF A NICKEL-INDUCIBLE CATION DIFFUSION FACILITATOR-NICOT COMBINED SYSTEM IN RHIZOBIUM LEGUMINOSARUM BV. VICIAE
Year:2012
Research Areas
  • Molecular, cellular and genetic biology
Information
Abstract
Analysis of Rhizobium leguminosarum bv. viciae strain 3841 genome sequence revealed a genetic system (RL1175 through RL1178), potentially involved in nickel homeostasis. The predicted RL1175 gene product shows sequence similarity to cation diffusion facilitator proteins, whereas RL1178 encodes a high-affinity nickel uptake transporter of the NiCoT family (nickel-cobalt transport) and the RL1176 gene product is an NreA-like nickel- responsive regulator. The confluence of uptake and efflux transport proteins potentially linked in the same genetic system resembles the organization of the ncrABC and nreBAC operons from Serratia marcenses and Legionella pneumophila, involved in nickel and cobalt resistance. Gene expression analysis using promoter fusions to lacZ and qRT-PCR assays revealed that the identified R. leguminosarum genes are organized as a single operon whose transcription is strongly induced by nickel, but not cobalt, ions in free-living cultures, as well as in pea bacteroid cells. Generation of mutants affected in this system is in progress and will hopefully help to define the role of the interplay between metal uptake and metal efflux systems in nickel resistance.
International
Si
Congress
10th European Nitrogen Fixation Conference
960
Place
Munich, Alemania
Reviewers
Si
ISBN/ISSN
00000-00000
Start Date
02/09/2012
End Date
05/09/2012
From page
23
To page
23
Abstract book
Participants
  • Autor: Rosa Isabel Prieto Carbajo (UPM)
  • Autor: Laura Rubio Sanz (UPM)
  • Autor: Silvia Burruezo (Universidad Politecnica de Madrid)
  • Autor: Juan Imperial Ródenas (UPM)
  • Autor: Jose Manuel Palacios Alberti (UPM)
  • Autor: Maria Belen Brito Lopez (UPM)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Asociaciones simbióticas planta-microorganismo
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología
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