Memorias de investigación
Artículos en revistas:
Distribution of indole-3-acetic acid in Petunia hybrida shoot tip cuttings and relationship between auxin transport, carbohydrate metabolism and adventitious root formation.
Año:2013
Áreas de investigación
  • Biología molecular, celular y genética
Datos
Descripción
To determine the contribution of polar auxin transport (PAT) to auxin accumulation and to adventitious root (AR) formation in the stem base of Petunia hybrida shoot tip cuttings, the level of indole-3-acetic acid (IAA) was monitored in non-treated cuttings and cuttings treated with the auxin transport blocker naphthylphthalamic acid (NPA) and was complemented with precise anatomical studies. The temporal course of carbohydrates, amino acids and activities of controlling enzymes was also investigated. Analysis of initial spatial IAA distribution in the cuttings revealed that approximately 40 and 10% of the total IAA pool was present in the leaves and the stem base as rooting zone, respectively. A negative correlation existed between leaf size and IAA concentration. After excision of cuttings, IAA showed an early increase in the stem base with two peaks at 2 and 24h post excision and, thereafter, a decline to low levels. This was mirrored by the expression pattern of the auxin-responsive GH3 gene. NPA treatment completely suppressed the 24-h peak of IAA and severely inhibited root formation. It also reduced activities of cell wall and vacuolar invertases in the early phase of AR formation and inhibited the rise of activities of glucose-6-phosphate dehydrogenase and phosphofructokinase during later stages. We propose a model in which spontaneous AR formation in Petunia cuttings is dependent on PAT and on the resulting 24-h peak of IAA in the rooting zone, where it induces early cellular events and also stimulates sink establishment. Subsequent root development stimulates glycolysis and the pentosephosphate pathway
Internacional
Si
JCR del ISI
Si
Título de la revista
Planta
ISSN
0032-0935
Factor de impacto JCR
3,35
Información de impacto
JCR
Volumen
238
DOI
10.1007/s00425-013-1907-z
Número de revista
3
Desde la página
499
Hasta la página
517
Mes
SEPTIEMBRE
Ranking
ISI Journal Citation Reports © Ranking: 2012: 28/195 (Plant Sciences)
Esta actividad pertenece a memorias de investigación
Participantes
  • Autor: Amir H. Ahkami Washington State University
  • Autor: Michael Melzer Leibniz Institute of Plant Genetics and Crop Plant Research
  • Autor: Mohammad R. Ghaffari Leibniz Institute of Plant Genetics and Crop Plant Research
  • Autor: Stephan Pollmann UPM
  • Autor: Fahimeh Shahinnia Leibniz Institute of Plant Genetics and Crop Plant Research
  • Autor: Majid Ghorbani University of Tehran
  • Autor: Mohammad R. Hajirezaei Leibniz Institute of Plant Genetics and Crop Plant Research
  • Autor: Uwe Druege Leibniz-Institute of Vegetable and Ornamental Crops Großbeeren/Erfurt e.V.
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Grupo de Investigación: Redes Reguladoras del Desarrollo y Respuesta a Estrés en Plantas