Observatorio de I+D+i UPM

Memorias de investigación
Research Publications in journals:
Building functional surfaces for biosensors development
Year:2013
Research Areas
  • Engineering
Information
Abstract
Polyelectrolyte multilayers (PEM) built by layer-by-layer technique have been extensively studied over the last years, resulting in a wide variety of current and potential applications. This technique can be used to construct thin films with different functionalities, or to functionalize surfaces with substantial different properties of those of the underlying substrates. The multilayering process is achieved by the alternate adsorption of oppositely charged polyelectrolytes. In this work we get advantage of the protein resistant property of the Poly(1-lysine)-graft(polyethyleneglycol) to create protein patterns. Proteins can be immobilized on a surface by unspecific physical adsorption, covalent binding or through specific interactions. The first protein used in this work was laccase, a copper-containing redox enzyme that catalyse the oxidation of a broad range of polyphenols and aromatic substrates, coupled to the reduction of O-2 to H2O without need of cofactors. Applications of laccases have been reported in food, pulp, paper, and textile industry, and also in biosensor development. Some uses require the immobilization of the enzyme on solid supports by adsorption, covalent attachment, entrapment, etc, on several substrates. Especially for biosensor development, highly active, stable and reproducible immobilization of laccase is required.
International
Si
JCR
Si
Title
Key Engineering Materials
ISBN
1013-9826
Impact factor JCR
0,224
Impact info
Datos JCR del año 2005
Volume
543
10.4028/www.scientific.net/KEM.543.204
Journal number
From page
204
To page
207
Month
SIN MES
Ranking
0
Participants
  • Autor: Carlos Moron Fernandez (UPM)
  • Autor: Alfonso Garcia Garcia (UPM)
  • Autor: Enrique Tremps Guerra (UPM)
  • Autor: Jose Andres Somolinos Sanchez (UPM)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Grupo de sensores y actuadores
  • Departamento: Tecnología de la Edificación
S2i 2019 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
Cofinanciación del MINECO en el marco del Programa INNPACTO (IPT-020000-2010-22)