Observatorio de I+D+i UPM

Memorias de investigación
Research Publications in journals:
Jasmonate-dependent modifications of the pectin matrix during potato development function as a defense mechanism targeted by Dickeya dadantii virulence factors
Year:2014
Research Areas
  • Molecular, cellular and genetic biology
Information
Abstract
The plant cell wall constitutes an essential protection barrier against pathogen attack. In addition, cell-wall disruption leads to accumulation of jasmonates (JAs), which are key signaling molecules for activation of plant inducible defense responses. However, whether JAs in return modulate the cell-wall composition to reinforce this defensive barrier remains unknown. The enzyme 13-allene oxide synthase (13-AOS) catalyzes the first committed step towards biosynthesis of JAs. In potato (Solanum tuberosum), there are two putative St13-AOS genes, which we show here to be differentially induced upon wounding. We also determine that both genes complement an Arabidopsis aos null mutant, indicating that they encode functional 13-AOS enzymes. Indeed, transgenic potato plants lacking both St13-AOS genes (CoAOS1/2 lines) exhibited a significant reduction of JAs, a concomitant decrease in wound-responsive gene activation, and an increased severity of soft rot disease symptoms caused by Dickeya dadantii. Intriguingly, a hypovirulent D. dadantii pel strain lacking the five major pectate lyases, which causes limited tissue maceration on wild-type plants, regained infectivity in CoAOS1/2 plants. In line with this, we found differences in pectin methyl esterase activity and cell-wall pectin composition between wild-type and CoAOS1/2 plants. Importantly, wild-type plants had pectins with a lower degree of methyl esterification, which are the substrates of the pectate lyases mutated in the pel strain. These results suggest that, during development of potato plants, JAs mediate modification of the pectin matrix to form a defensive barrier that is counteracted by pectinolytic virulence factors from D. dadantii.
International
Si
JCR
Si
Title
Plant Journal
ISBN
0960-7412
Impact factor JCR
6,582
Impact info
Datos JCR del año 2012
Volume
77
10.1111/tpj.12393
Journal number
From page
418
To page
429
Month
SIN MES
Ranking
Participants
  • Autor: Marco Taurino (Centro Nacional de Biotecnologia)
  • Autor: Jose Abelenda (Centro Nacional de Biotecnologia)
  • Autor: Isabel Del Rio Alvarez (UPM)
  • Autor: C Navarro (Centro Nacional de Biotecnologia)
  • Autor: B Vicedo (Centro Nacional de Biotecnologia)
  • Autor: T Farmaki (centro Nacional de Biotecnologia)
  • Autor: P Jimenez (Centro Nacional de Biotecnología)
  • Autor: P García Agustin (Centro Nacional de Biotecnologia)
  • Autor: Emilia Antonia Lopez Solanilla (UPM)
  • Autor: Salome Prat (Centro Nacional de Biotecnologia)
  • Autor: Enrique Rojo (Centro Nacional de Biotecnologia)
  • Autor: Jose Sanchez Serrano (Centro Nacional de Biotecnologia)
  • Autor: Maite Sanmartin (Centro Nacional de Biotecnología)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Interacciones Moleculares Planta-Patógeno
  • Departamento: Biotecnología - Biología Vegetal
S2i 2020 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
Cofinanciación del MINECO en el marco del Programa INNPACTO (IPT-020000-2010-22)