Observatorio de I+D+i UPM

Memorias de investigación
Communications at congresses:
Mitochondrial AtTRXo1: its transcriptional regulation and role under salt-stress (póster)
Year:2013
Research Areas
  • Molecular biology,
  • Agronomic research
Information
Abstract
Thioredoxins (Trxs) are ubiquitous small proteins involved in the reduction of disulfide bonds of other proteins in different cell compartments. Among them, mitochondrial Trxo has been described to have a response in plants grown under salinity but there is scarce information about its functional role in abiotic stress or its gene regulation. In this work, the transcriptional regulation of the mitochondrial AtTrxo1 gene has been studied for the first time, by producing severa! AtTrxo1::GUS transgenic Arabidopsis plants containing conserved domains chosen comparing Brasicacea Trxo1 promoters. Three motifs were found as possible positive regulators and one as negative of AtTrxo1 expression. Using an arrayed yeast library from A. thaliana, a set of transcription factors were shown as prey of AtTrxo1 prometer, and among them, we underlined Bzip9 and AZF2, both strongly related with biotic and abiotic stress, respectively. We have also studied the response of mutant AtTrxo1 plants to salinity, in arder to gain insight on its functional role under stress. For it, a physiological characterization of the mutant and the study on its germination pattern was carried out. Results indicated that the lack of AtTrxo1 produced phenotypic changes and influenced the time to 50% germination under salinity, without affecting the final germination percentage. Results are discussed in relation to Trxo1 expression pattern and its regulation.
International
No
Congress
VIII REUNIÓN del GRUPO ESPAÑOL DE INVESTIGACIÓN EN RADICALES LIBRES {GEIRLI)
960
Place
Valencia
Reviewers
Si
ISBN/ISSN
0000-0000
Start Date
03/06/2013
End Date
05/06/2013
From page
59
To page
59
VIII REUNIÓN del GRUPO ESPAÑOL DE INVESTIGACIÓN EN RADICALES LIBRES {GEIRLI)
Participants
  • Autor: Ana María Ortíz Espín (CEBAS-CSIC)
  • Autor: Raquel Iglesias Fernandez (UPM)
  • Autor: Aingeru Calderón (CEBAS-CSIC)
  • Autor: Francisca Sevilla (CEBAS-CSIC)
  • Autor: Pilar Carbonero Zalduegui (UPM)
  • Autor: Ana Jiménez (CEBAS-CSIC)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Biotecnología y Genómica de Semillas
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología
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