Observatorio de I+D+i UPM

Memorias de investigación
Communications at congresses:
Characterization of HupE, a nickel transporter for hydrogenase synthesis in the endosymbiotic bacterium Rhizobium leguminosarum bv. viciae.
Year:2013
Research Areas
  • Bacteriology
Information
Abstract
Rhizobium leguminosarumbv. viciae (Rlv) UPM791 synthesizes a NiFe hydrogenase that allows the recycling of hydrogen produced by nitrogenase. In this bacterium, the synthesis of hydrogenase requires the participation of 18 proteins encoded in the hup/hyp gene cluster hupSLCDE FGHIJKhypABF CDEX located in the symbiotic plasmid. One of the genes of this cluster, hupE , encodes a protein harboring a canonical signal peptide and six potential transmembrane domains. Previous hydrogenase activity and Ni63 transport assays demonstrated that HupE is a nickel transporter that provides this metal for the synthesis of hydrogenase in Rlv UPM791 free-living cells and lentil bacteroids, and led to the identification of two histidine-rich motifs required for HupE functionality (Brito et al., 2010). A similar protein (UreJ) is present in gene clusters involved in urease synthesis in other bacteria. HupE/UreJ constitutes a family of single permeases widely distributed in bacteria. The characterization of kinetic and functional properties of Rlv UPM791 HupE is being carried out through heterologous expression of HupE in a nik -deficient Escherichia coli background. The specificity of this transporter for Ni2+ is being determined in competition assays with other divalent metal ions. A HupE variant lacking the N-terminal periplasmic region of the mature protein has been constructed to ascertain the potential role of this domain in ion scavenging and concentration around the transporter. The relevance of other residues conserved in members of the HupE/UreJ family is being tested through the analysis of site-specific mutants. The results of the on-going work will be presented and discussed.
International
Si
Congress
10th International Hydrogenase Conference
960
Place
Szeged, Hungría
Reviewers
Si
ISBN/ISSN
0000-0000
Start Date
08/07/2013
End Date
12/07/2013
From page
143
To page
143
Book of Abstracts
Participants
  • Autor: Marta Albareda Contreras (UPM)
  • Autor: Marie Andree Mandrand-Berthelot (Universidad de Lyon)
  • Autor: Agnes Rodrigue (Universidad de Lyon)
  • Autor: Tomas-Andres Ruiz Argueso (UPM)
  • Autor: Juan Imperial Ródenas (UPM)
  • Autor: Jose Manuel Palacios Alberti (UPM)
  • Autor: Maria Belen Brito Lopez (UPM)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Asociaciones simbióticas planta-microorganismo
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología
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