Observatorio de I+D+i UPM

Memorias de investigación
Communications at congresses:
Characterization of HupE, a nickel transporter for hydrogenase synthesis in the endosymbiotic bacterium Rhizobium leguminosarum bv. viciae.
Research Areas
  • Bacteriology
Rhizobium leguminosarumbv. viciae (Rlv) UPM791 synthesizes a NiFe hydrogenase that allows the recycling of hydrogen produced by nitrogenase. In this bacterium, the synthesis of hydrogenase requires the participation of 18 proteins encoded in the hup/hyp gene cluster hupSLCDE FGHIJKhypABF CDEX located in the symbiotic plasmid. One of the genes of this cluster, hupE , encodes a protein harboring a canonical signal peptide and six potential transmembrane domains. Previous hydrogenase activity and Ni63 transport assays demonstrated that HupE is a nickel transporter that provides this metal for the synthesis of hydrogenase in Rlv UPM791 free-living cells and lentil bacteroids, and led to the identification of two histidine-rich motifs required for HupE functionality (Brito et al., 2010). A similar protein (UreJ) is present in gene clusters involved in urease synthesis in other bacteria. HupE/UreJ constitutes a family of single permeases widely distributed in bacteria. The characterization of kinetic and functional properties of Rlv UPM791 HupE is being carried out through heterologous expression of HupE in a nik -deficient Escherichia coli background. The specificity of this transporter for Ni2+ is being determined in competition assays with other divalent metal ions. A HupE variant lacking the N-terminal periplasmic region of the mature protein has been constructed to ascertain the potential role of this domain in ion scavenging and concentration around the transporter. The relevance of other residues conserved in members of the HupE/UreJ family is being tested through the analysis of site-specific mutants. The results of the on-going work will be presented and discussed.
10th International Hydrogenase Conference
Szeged, Hungría
Start Date
End Date
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Book of Abstracts
  • Autor: Marta Albareda Contreras (UPM)
  • Autor: Marie Andree Mandrand-Berthelot (Universidad de Lyon)
  • Autor: Agnes Rodrigue (Universidad de Lyon)
  • Autor: Tomas-Andres Ruiz Argueso (UPM)
  • Autor: Juan Imperial Ródenas (UPM)
  • Autor: Jose Manuel Palacios Alberti (UPM)
  • Autor: Maria Belen Brito Lopez (UPM)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Asociaciones simbióticas planta-microorganismo
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología
S2i 2020 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
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