Memorias de investigación
Ponencias en congresos:
Nitrogen fixation by native Bradyrhizobia in symbiosis with Lupinus mariae-josephae requires a T3SS encoding a NopE-like effector
Año:2013
Áreas de investigación
  • Bacteriología
Datos
Descripción
Several bradyrhizobial isolates from L. mariae-josephae root nodules [1] contain a type III secretion system (T3SS) within a cluster of about 30 genes. Among those genes, ttsI codes for the transcriptional activator of the system. Mutation of ttsI resulted in the formation of white, non-fixing nodules with the natural legume host, L. mariae-josephae. The T3SS cluster also contains a gene coding for a NopE-like protein. NopE proteins have been demonstrated to be effectors in the Bradyrhizobium-soybean symbiosis [2] and belong to a small group of poorly characterized proteins from plant-associated bacteria that contain one or two autocleavage motifs known as DUF1521 (Schirrmeister et al. 2011). The amino acid sequence of a NopE-like protein in the L. mariae-josephae strain LmjC contains just one autocatalytic motif. This is unlike NopE1 and NopE2 proteins secreted by the T3SS of B. japonicum, that contain two motifs [3]. The autocleavage of LmjC NopE protein was analyzed after expression in E. coli and purification. Two protein fragments of the predicted sizes appeared in the presence of Ca2+, Cu2+, Cd2+, Zn2+ and Mn2+ cations. In contrast, autocleavage did not take place in the presence of Ni2+, Co2+ or Mg2+. Site-directed mutagenesis of the DUF1521 motif in LmjC NopE abolished self-cleavage in vitro. Symbiotic competence of a NopE- mutant with the L. mariae-josephae host was not affected. Possible roles of NopE are discussed.
Internacional
No
Nombre congreso
XXXVI Congreso SEBBM
Tipo de participación
960
Lugar del congreso
Madrid
Revisores
Si
ISBN o ISSN
0000-0000
DOI
Fecha inicio congreso
04/09/2013
Fecha fin congreso
06/09/2013
Desde la página
254
Hasta la página
255
Título de las actas
Book of Abstracts
Esta actividad pertenece a memorias de investigación
Participantes
  • Autor: David Ricardo Duran Wendt UPM
  • Autor: Victor Pastor UPM (Alumno)
  • Autor: Susanne Zehner, TU Dresden, Institute of Genetics, Dresden, Germany
  • Autor: Michael Göttfert, TU Dresden, Institute of Genetics, Dresden, Germany
  • Autor: Juan Imperial Ródenas UPM
  • Autor: Tomas-Andres Ruiz Argueso UPM
  • Autor: Luis Rey Navarro UPM
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Grupo de Investigación: Asociaciones simbióticas planta-microorganismo
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología