Memorias de investigación
Artículos en revistas:
The type II secretion system (Xcp) of Pseudomonas putida is active and involved in the secretion of phosphatases.
Año:2013
Áreas de investigación
  • Agricultura
Datos
Descripción
The genome of the Gram-negative bacterium Pseudomonas putida harbours a complete set of xcp genes for a type II protein secretion system (T2SS). This study shows that expression of these genes is induced under inorganic phosphate (Pi ) limitation and that the system enables the utilization of various organic phosphate sources. A phosphatase of the PhoX family, previously designated UxpB, was identified, which was produced under low Pi conditions and transported across the cell envelope in an Xcp-dependent manner demonstrating that the xcp genes encode an active T2SS. The signal sequence of UxpB contains a twin-arginine translocation (Tat) motif as well as a lipobox, and both processing by leader peptidase II and Tat dependency were experimentally confirmed. Two different tat gene clusters were detected in the P.?putida genome, of which one, named tat-1, is located adjacent to the uxpB and xcp genes. Both Tat systems appeared to be capable of transporting the UxpB protein. However, expression of the tat-1 genes was strongly induced by low Pi levels, indicating a function of this system in survival during Pi starvation.
Internacional
Si
JCR del ISI
Si
Título de la revista
Environmental Microbiology
ISSN
1462-2912
Factor de impacto JCR
5,756
Información de impacto
Volumen
15
DOI
10.1111/1462-2920.12115
Número de revista
10
Desde la página
2658
Hasta la página
2671
Mes
OCTUBRE
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Participantes
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Grupo de Investigación: Interacciones Moleculares Planta-Patógeno
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología