Observatorio de I+D+i UPM

Memorias de investigación
Research Publications in journals:
Cytisus villosus from Northeastern Algeria is nodulated by genetically diverse Bradyrhizobium strains
Year:2013
Research Areas
  • Microbiology
Information
Abstract
Fifty-one rhizobial strains isolated from root nodules of Cytisus villosus growing in Northeastern Algeria were characterized by genomic and phenotypic analyses. Isolates were grouped into sixteen different patterns by PCR-RAPD. The phylogenetic status of one representative isolate from each pattern was examined by multilocus sequence analyses of four housekeeping genes (16S rRNA, glnII, recA, and atpD) and one symbiotic gene (nodC). Analysis of 16S rRNA gene sequences showed that all the isolates belonged to the genus Bradyrhizobium. Phylogenetic analyses based on individual or concatenated genes glnII, recA, and atpD indicated that strains cluster in three distinct groups. Ten out of the sixteen strains grouped together with Bradyrhizobium japonicum, while a second group of four clustered with Bradyrhizobium canariense. The third group, represented by isolates CTS8 and CTS57, differed significantly from all other bradyrhizobia known to nodulate members of the Genisteae tribe. In contrast with core genes, sequences of the nodC symbiotic gene from all the examined strains form a homogeneous group within the genistearum symbiovar of Bradyrhizobium. All strains tested nodulated Lupinus angustifolius, Lupinus luteus, and Spartium junceum but not Glycine max. From these results, it is concluded that C. villosus CTS8 and CTS57 strains represent a new lineage within the Bradyrhizobium genus.
International
Si
JCR
Si
Title
Antonie Van Leeuwenhoek International Journal of General And Molecular Microbiology
ISBN
0003-6072
Impact factor JCR
2,072
Impact info
Volume
10.1007/s10482-014-0173-9
Journal number
From page
1121
To page
1129
Month
SIN MES
Ranking
Participants
  • Autor: Hadjira Ahnia (Laboratoire d¿Ecologie Microbienne, Faculte¿ des Sciences de la Nature et de la Vie, Universite¿ de Bejaia,)
  • Autor: Farida Boulila (Laboratoire d¿Ecologie Microbienne, Faculte¿ des Sciences de la Nature et de la Vie, Universite¿ de Bejaia)
  • Autor: Abdelghani Boulila (Laboratoire d¿Ecologie Microbienne, Faculte¿ des Sciences de la Nature et de la Vie, Universite¿ de Bejaia)
  • Autor: Karima Boucheffa (Laboratoire d¿Ecologie Microbienne, Faculte¿ des Sciences de la Nature et de la Vie, Universite¿ de Bejaia)
  • Autor: David Ricardo Duran Wendt (UPM)
  • Autor: Yasmina Bourebaba (Laboratoire d¿Ecologie Microbienne, Faculte¿ des Sciences de la Nature et de la Vie, Universite¿ de Bejaia)
  • Autor: Adouda Salmi (Laboratoire d¿Ecologie Microbienne, Faculte¿ des Sciences de la Nature et de la Vie, Universite¿ de Bejaia)
  • Autor: Juan Imperial Ródenas (UPM)
  • Autor: Tomas-Andres Ruiz Argueso (UPM)
  • Autor: Luis Rey Navarro (UPM)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Asociaciones simbióticas planta-microorganismo
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología
S2i 2020 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
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