Observatorio de I+D+i UPM

Memorias de investigación
Research Publications in journals:
SEQUENTIAL STUDY OF THE GENETIC STABILITY OF CALLUS AND REGENERATED SHOOTS IN CHRYSANTHEMUM
Year:2014
Research Areas
  • Conservation biology,
  • Crop production
Information
Abstract
Callus and adventitious shoots were induced on chrysanthemum ?Red Reagan? leaves following a protocol with different steps in which several factors were studied: callus induction (two variants of MS medium and light/ darkness), shoot regeneration (two variants of the medium), and shoot elongation and rooting (three variants of the medium). The influence of the different factors on the response observed and on the genetic stability was studied throughout the process. Genetic stability was assessed using RAPD markers. The identity of the samples was recorded throughout the entire process. The incubation conditions (light/darkness) did have a significant effect on the amount of callus produced. Shoot production in the regeneration phase was not influenced by the incubation conditions or variants of the medium used neither in the previous step (callus induction) nor by the medium used to induce shoot development. Regarding the genetic stability study, all shoots regenerated showed identical RAPD marker profile to those of the pot stock plant (control) and to those of the in vitro shoots from which leaves were used as explants. However, 32% of the calluses sampled after the callus induction step and 23% of those sampled after the shoot induction step were genetically different to the control. From the same explant, stable shoots and variant callus were obtained, which indicates a selection of cells that form organized growth during the process.
International
Si
JCR
Si
Title
Propagation of Ornamental Plants
ISBN
1311-9109
Impact factor JCR
0,351
Impact info
Datos JCR del año 2013
Volume
14
Journal number
From page
57
To page
67
Month
SIN MES
Ranking
Participants
  • Autor: Hilda Soledad Miñano (Universidad Politécnica de Madrid)
  • Autor: Miguel Angel Ibañez Ruiz (UPM)
  • Autor: M. Elena Gonzalez Benito (UPM)
  • Autor: Maria Carmen Martin Fernandez (UPM)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Biodiversidad y conservación de recursos fitogenéticos
  • Departamento: Economía Agraria, Estadística y Gestión de Empresas
  • Departamento: Biotecnología - Biología Vegetal
S2i 2020 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
Cofinanciación del MINECO en el marco del Programa INNPACTO (IPT-020000-2010-22)