Observatorio de I+D+i UPM

Memorias de investigación
Communications at congresses:
Study on recombination among Soybean mosaic virus isolates
Year:2014
Research Areas
  • Phytopathology,
  • Viral pathogens of plants
Information
Abstract
Soybean mosaic virus (SMV), a member of the genus Potyvirus, is one of the most important viral diseases of soybean in all soybean-producing regions worldwide. In 2012, some SMV isolates were collected from Mazandaran, Golestan, Ardabil and Lorestan provinces in Iran. Full length genome sequences of four isolates and nucleotide sequence of P1, P3, 6K1, CI and CP of 21 other SMV isolates were determined. Recombination events, most likely parental isolates of recombinants and recombination break points were identified by RDP, GENECONV, Chimaera, MaxChi, BOOTSCAN and SISCAN methods as implemented in RDP4 Beta 4.16. Recombination was analyzed among Iranian SMV isolates plus a set of 52 SMV isolates from other parts of the world, for which the whole length genome sequence was reported in databases. Either full-length genome sequences or the concatenated sequences encoding proteins P1, P3, 6K1, CI and CP were analyzed. Recombination analysis showed that the genomes of most reported SMV isolates (55 out 77 isolates) have evidences of recombination events. The inclusion of the Iranian SMV isolates in this analysis allowed to identify new recombination sites in the SMV genome, in addition to those reported previously. Recombination events were also detected between different pathotypes, and thus provided evidence that distinct SMV pathotypes can simultaneously infect a host cell. Accumulating evidence suggests that recombination is a common and important source of genetic variation and evolution in SMV.
International
Si
Congress
21st Iranian Plant Protection Congress
960
Place
Urmia University
Reviewers
Si
ISBN/ISSN
000-00-0000-000-0
Start Date
23/09/2014
End Date
26/09/2014
From page
75
To page
75
Healthy plants in our hands
Participants
  • Autor: Akbar Ahangaran (University of Tehran)
  • Autor: M. Koohi Habibi (University of Tehran)
  • Autor: GH. Masahebi Mohamadi (University of Tehran)
  • Autor: Fernando Garcia-Arenal Rodriguez (UPM)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Patología Vegetal
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología - Biología Vegetal
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