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Descripción
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| In the Soybean mosaic virus (SMV)- soybean pathosystem, three independent genes (Rsv1, Rsv3 and Rsv4) conferring resistance to SMV have been identified. Previously, role of Q1033K and G1054R substitutions in the P3 cistron of avirulent isolates, individually or in combination, in virulence on V94-5152 (Rsv4) was identified. In 2012, 25 SMV isolates were collected from main soybean production areas for identification of SMV strains in Iran. Seven soybean resistant cultivars with specific resistance genes were used for differentiation of strains. The inoculation of fifteen Iranian SMV isolates on resistant cultivars carrying Rsv1 resistance alleles showed none had evolved virulence. All isolates had evolved virulence towards L29 (Rsv3) and out of 15 isolates, 13 isolates infected resistant cultivar V94-5152 (Rsv4). Alignment of P3 amino acid sequences of Iranian isolates showed that difference between virulent and avirulent Iranian isolates on Rsv4-genotype was at polypeptide position 1053, where amino acid serine (S) in avirulent isolate was replaced by asparagine (N) in virulent isolates (S1053N). To determine whether S1053N substitution is sufficient to confer virulence to avirulent isolate SMV-Ar33 on V94-5152 (Rsv4), PCR- based site-directed mutagenesis was performed for constructing pSMV-Ar33S1053N. Interestingly, pSMV-Ar33S1053N infected Rsv4 resistance cultivar V94-5152. So, this amino acids substitution may be involved in breaking Rsv4 resistance. Our results with previous studies suggest that a single amino acid change in C-terminal region of P3 is Rsv4-breaking determinant in the SMV-soybean pathosystem. | |
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Internacional
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Si |
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Nombre congreso
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21st Iranian Plant Protection Congress |
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Tipo de participación
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960 |
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Lugar del congreso
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Urmia University, Iran |
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Revisores
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Si |
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ISBN o ISSN
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000-00-0000-000-0 |
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DOI
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Fecha inicio congreso
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23/09/2014 |
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Fecha fin congreso
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26/09/2014 |
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Desde la página
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317 |
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Hasta la página
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317 |
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Título de las actas
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Healthy Plant in our Hands |