Observatorio de I+D+i UPM

Memorias de investigación
Research Project:
PLASWIRES: Engineering multicellular biocircuits: programming cell-cell communication using plasmids as wires
Year:2013
Research Areas
Information
Abstract

The goal of PLASWIRE project is to demonstrate (empirically, theoretically, and computationally) that conjugation is a modular, robust and local cell-cell communication mechanism that allows the engineering of scalable, programmable, versatile, and distributed living plasmid technology with potential applications in: Computer science and synthetic biology, Biomedicine, nanobiotechnology and biochemical engineering.
The main scientific and technological achievements of the project will be:


A. The engineering of the following multicellular plasmid based genetic systems:

1. Sentinel plasmids that will serve as new programmable antibiotics able to detect dangerous combinations of plasmids.
2. The largest multicellular genetic circuits engineered so far:
2.1 A multi-layered AND gate circuit with 5 inputs
2.2 AND-OR and OR-AND Boolean logic gates circuits with up to 6 inputs
3. A programmable sensor of 2 plasmids (if-then-else).
4. A multicellular Programmable Logic Array (PLA) of 2 inputs.
5. “Clause plasmids” able to solve autonomously in linear time the SAT problem in bacterial populations


B. The design and simulation of other multicellular plasmid based circuits: Edge-detector multicellular plasmid circuits, smart task scheduler plasmids, foreman plasmids, and predator-prey multicellular circuits.
C. Theoretical analysis of the computational power of distributed communication plasmid protocols and
D. The development of a software conjugative module to the CellModeller and BactoSIM simulators.

International
Si
Project type
Proyectos y convenios en convocatorias públicas competitivas
Company
Entity Nationality
Sin nacionalidad
Entity size
Granting date
22/07/2013
Participants
  • Participante: Ivan Pau De la Cruz (UPM)
  • Participante: Petr Sosik (UPM)
  • Participante: Paul Andrei Paun (UPM)
  • Director: Alfonso Vicente Rodriguez-Paton Aradas (UPM)
  • Participante: Martín Gutiérrez Pescarmona (UPM)
  • Participante: Guillermo Pérez del Pulgar Frowein (UPM)
  • Participante: Paula Gregorio Godoy (UPM)
  • Participante: María Esther Pozuelo De Prada (UPM)
  • Participante: Juan Diaz Colunga (UPM)
Research Group, Departaments and Institutes related
  • Creador: No seleccionado
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