Observatorio de I+D+i UPM

Memorias de investigación
Communications at congresses:
Colletotrichum tofieldiae is a root-infecting endophyte on Arabidopsis and promotes plant growth under phosphate-limiting conditions
Year:2014
Research Areas
  • Soil biology,
  • Crop production
Information
Abstract
Colletotrichum species are generally considered phytopathogenic fungi, causing anthracnose diseases on many plant species, including Arabidopsis. However, Colletotrichum fungi are also frequently isolated as endophytes from plants without visible symptoms after surface sterilization. Here, we focused on one such endophytic strain, C. tofieldiae (Ct), which was isolated from a wild Arabidopsis population in Spain. To track the infection process in planta, we used Agrobacterium mediated transformation to generate transgenic Ct lines expressing green fluorescent protein (GFP). After inoculating conidia onto the roots of Arabidopsis plants grown in vitro, we found Ct initially penetrates by means of undifferentiated germ-tubes and then extensively colonizes root tissues as both inter- and intracellular hyphae. At 28 days after root inoculation, we detected GFP-expressing hyphae inside leaves without symptoms, suggesting Ct is an endophytic fungus that can colonize Arabidopsis systemically without causing disease. Remarkably, Ct infection promoted plant growth under phosphate-limiting conditions, as measured by enhanced shoot fresh-weight and root length. In contrast, a Colletotrichum species belonging to the same taxonomic clade as Ct, which causes disease on Japanese radish, severely inhibited growth of Arabidopsis under the same conditions. Because these two fungi are closely-related species, our results suggest that their endophytic and pathogenic life-styles may be determined by subtle genetic differences. We have therefore initiated comparative genomic and transcriptomic analyses of these strains to uncover the molecular signatures underlying endophytism and the origin of pathogenicity in Colletotrichum.
International
Si
Congress
XVI International Congress on Molecular Plant-Microbe Interactions
960
Place
Rodas (Grecia)
Reviewers
Si
ISBN/ISSN
000-00-0000-000-0
Start Date
06/07/2014
End Date
10/07/2014
From page
11
To page
11
XVI International Congress on Molecular Plant-Microbe Interactions
Participants
  • Autor: Kei Hiruma (Max Planck Institute for Plant Breeding Research)
  • Autor: Barbara Kracher (Max Planck Institute for Plant Breeding Research)
  • Autor: Emiel Ver Loren van Themaat (Max Planck Institute for Plant Breeding Research)
  • Autor: Ryohei Thomas Nakano (Max Planck Institute for Plant Breeding Research)
  • Autor: Ulla Neumann (Max Planck Institute for Plant Breeding Research)
  • Autor: Nina Gerlach (University of Cologne)
  • Autor: Marcel Bucher (University of Cologne)
  • Autor: Stephane Hacquard (Max Planck Institute for Plant Breeding Research)
  • Autor: Soledad Sacristan Benayas (UPM)
  • Autor: Richard O' Connell (INRA-AgroParisTech)
  • Autor: Paul Schulze-Lefert (Max Planck Institute for Plant Breeding Researc)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Patología Vegetal
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología - Biología Vegetal
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