Observatorio de I+D+i UPM

Memorias de investigación
Research Publications in journals:
The role of N-glycosilation in kiwi allergy
Year:2014
Research Areas
  • Molecular mechanism of disease
Information
Abstract
The physical, biochemical, and immunological characteristics of plant allergens have been widely studied, but no definite conclusion has been reached about what actually makes a protein an allergen. In this sense, N-glycosylation is an exclusive characteristic of plant allergens not present in mammals and it could be implied in allergenic sensitization. With this aim, we evaluated and compared the allergenic activity of the protein fraction and the N-glycan fraction of the thaumatin-like protein and the main kiwi allergen, Act d 2. The natural allergen, Act d 2, was deglycosylated by trifluoromethanesulfonic acid treatment; the N-glycan fraction was obtained by extended treatment with proteinase K. N-glycan- and protein- fractions were recognized by specific IgE of kiwi-allergic patients. By contrast, the sugar moiety showed a reduced capacity to activate basophils and T cells, but not dendritic cells derived from patients? monocytes. Related to this, the production of cytokines such as IL6 and IL10 was increased by the incubation of dendritic cells with sugar moiety. Thus, the sugar moiety plays a significant role in sensitization, inducing the activation of antigen-presenting cells, but it is the protein fraction that is responsible for the allergic reactions.
International
No
JCR
No
Title
Food Science & Nutrition
ISBN
2048-7177
Impact factor JCR
Impact info
Volume
3
Journal number
From page
260
To page
271
Month
MAYO
Ranking
Participants
  • Autor: Maria Garrido Arandia (UPM)
  • Autor: Cristina Gomez Casado (UPM)
  • Autor: Amaya Murua Garcia (UPM)
  • Autor: Arantxa Palacin
  • Autor: Leticia Tordesillas (UPM)
  • Autor: Natalia Blanca-López
  • Autor: Tania Ramos
  • Autor: Gabriela Canto
  • Autor: Javier Cuesta-Herranz
  • Autor: Rosa Sánchez-Monge (UPM)
  • Autor: Luis Fernandez Pacios (UPM)
  • Autor: Araceli Diaz Perales (UPM)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Biotecnología Vegetal
  • Departamento: Sistemas y Recursos Naturales
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología - Biología Vegetal
S2i 2020 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
Cofinanciación del MINECO en el marco del Programa INNPACTO (IPT-020000-2010-22)