Observatorio de I+D+i UPM

Memorias de investigación
Research Publications in journals:
Immune Suppressive Effect of Cinnamaldehyde Due to Inhibition of Proliferation and Induction of Apoptosis in Immune Cells: Implications in Cancer
Year:2014
Research Areas
  • Biomedicine
Information
Abstract
Background: Besides its anti-inflammatory effects, cinnamaldehyde has been reported to have anti-carcinogenic activity. Here, we investigated its impact on immune cells. Methods: Activation of nuclear factor-kB by cinnamaldehyde (0?10 mg/ml) alone or in combination with lipopolysaccharide was assessed in THP1XBlue human monocytic cell line and in human peripheral blood mononuclear cells (PBMCs). Proliferation and secretion of cytokines (IL10 and TNFa) was determined in primary immune cells and the human cell lines (THP1, Jurkat E6-1 and Raji cell lines) stimulated with cinnamaldehyde alone or in conjunction with lipopolysaccharide. Nitric oxide was determined in mouse RAW264.7 cells. Moreover, different treated PBMCs were stained for CD3, CD20 and AnnexinV. Results: Low concentrations (up to 1 mg/ml) of cinnamaldehyde resulted in a slight increase in nuclar factor-kB activation, whereas higher concentrations led to a dose-dependent decrease of nuclear factor-kB activation (up to 50%) in lipopolysachharide-stimulated THP1 cells and PBMCs. Accordingly, nitric oxide, interleukin 10 secretion as well as cell proliferation were reduced in lipopolysachharide-stimulated RAW264.7 cells, PBMCs and THP1, Raji and Jurkat-E6 immune cells in the presence of cinnamaldehyde in a concentration-dependent manner. Flow cytometric analysis of PBMCs revealed that CD3+ were more affected than CD20+ cells to apopotosis by cinnamaldehyde. Conclusion: We attribute the anti-inflammatory properties of cinnamaldehyde to its ability to block nuclear factor-kB activation in immune cells. Treatment with cinnamaldehyde led to inhibition of cell viability, proliferation and induced apoptosis in a dose-dependent manner in primary and immortalized immune cells. Therefore, despite its described anticarcinogenic property, treatment with cinnamaldehyde in cancer patients might be contraindicated due to its ability to inhibit immune cell activation.
International
Si
JCR
Si
Title
Plos One
ISBN
1932-6203
Impact factor JCR
3,534
Impact info
Volume
10.1371/journal.pone.0108402
Journal number
From page
1
To page
7
Month
SIN MES
Ranking
Participants
  • Autor: F Roth-Walter
  • Autor: A Moskovskich
  • Autor: Cristina Gomez Casado (UPM)
  • Autor: Araceli Diaz Perales (UPM)
  • Autor: K Oida
  • Autor: J Singer
  • Autor: T Kinaciyan
  • Autor: HC Fuchs
  • Autor: E Jensen-Jarolim1
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Biotecnología Vegetal
S2i 2020 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
Cofinanciación del MINECO en el marco del Programa INNPACTO (IPT-020000-2010-22)