Memorias de investigación
Artículos en revistas:
Improved walnut mass micropropagation through the combined use of phloroglucinol and FeEDDHA
Año:2015
Áreas de investigación
  • Biología molecular, celular y genética
Datos
Descripción
Despite the socioeconomic importance of walnut trees, poor rooting and recalcitrance to in vitro culture have hampered the establishment of high-yield clonal plantations. To improve walnut micropropagation, we introduced several modifications to current methods and evaluated the effects on microshoot performance and acclimatization. Nine selected genotypes (13-year-old trees) of the commercial hybrid Juglans major 209 x J. regia were cultured in vitro on DKW-C medium supplemented with 4.4 µM BA and 50 µM IBA. A protocol was developed that relies on the use of 0.40 mM phloroglucinol during shoot multiplication, 0.20 mM previous root induction, and 6.81 mg/L Fe3+ (FeEDDHA). Moreover, the addition of 83.2 µM glucose during the root expression phase significantly improved plant survival during acclimatization. Phloroglucinol promoted microshoot elongation but inhibited rooting, especially at concentrations above 0.40 mM. Replacing FeEDTA by FeEDDHA diminished chlorotic symptoms and improved rooting, with up to 90% microshoots developing viable roots. Likewise, glucose was more efficient than sucrose or fructose in promoting plant survival. At the proposed working concentrations, neither glucose nor FeEDDHA caused any noticeable deleterious effect on walnut micropropagation. Microscopic analysis revealed the physical continuity between adventitious roots and stem pericycles. Analysis of leaf genomic DNA with eight polymorphic microsatellite markers was supportive of the clonal fidelity and genetic stability of the micropropagated material. Successful clonal plantations (over 5,800 ramets) have been established by applying this protocol.
Internacional
Si
JCR del ISI
Si
Título de la revista
Plant Cell Tissue And Organ Culture
ISSN
0167-6857
Factor de impacto JCR
2,612
Información de impacto
Datos JCR del año 2013
Volumen
123
DOI
DOI 10.1007/s11240-015-0822-3
Número de revista
1
Desde la página
143
Hasta la página
154
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SIN MES
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  • Creador: Departamento: Sistemas y Recursos Naturales