Observatorio de I+D+i UPM

Memorias de investigación
Research Publications in journals:
Improved walnut mass micropropagation through the combined use of phloroglucinol and FeEDDHA
Year:2015
Research Areas
  • Molecular, cellular and genetic biology
Information
Abstract
Despite the socioeconomic importance of walnut trees, poor rooting and recalcitrance to in vitro culture have hampered the establishment of high-yield clonal plantations. To improve walnut micropropagation, we introduced several modifications to current methods and evaluated the effects on microshoot performance and acclimatization. Nine selected genotypes (13-year-old trees) of the commercial hybrid Juglans major 209 x J. regia were cultured in vitro on DKW-C medium supplemented with 4.4 µM BA and 50 µM IBA. A protocol was developed that relies on the use of 0.40 mM phloroglucinol during shoot multiplication, 0.20 mM previous root induction, and 6.81 mg/L Fe3+ (FeEDDHA). Moreover, the addition of 83.2 µM glucose during the root expression phase significantly improved plant survival during acclimatization. Phloroglucinol promoted microshoot elongation but inhibited rooting, especially at concentrations above 0.40 mM. Replacing FeEDTA by FeEDDHA diminished chlorotic symptoms and improved rooting, with up to 90% microshoots developing viable roots. Likewise, glucose was more efficient than sucrose or fructose in promoting plant survival. At the proposed working concentrations, neither glucose nor FeEDDHA caused any noticeable deleterious effect on walnut micropropagation. Microscopic analysis revealed the physical continuity between adventitious roots and stem pericycles. Analysis of leaf genomic DNA with eight polymorphic microsatellite markers was supportive of the clonal fidelity and genetic stability of the micropropagated material. Successful clonal plantations (over 5,800 ramets) have been established by applying this protocol.
International
Si
JCR
Si
Title
Plant Cell Tissue And Organ Culture
ISBN
0167-6857
Impact factor JCR
2,612
Impact info
Datos JCR del ańo 2013
Volume
123
DOI 10.1007/s11240-015-0822-3
Journal number
1
From page
143
To page
154
Month
SIN MES
Ranking
Participants
  • Autor: Ricardo Licea . (UPM)
  • Autor: Ángela Bibiana Contreras Mogollon (UPM)
  • Autor: Luis Gomez Fernandez (UPM)
Research Group, Departaments and Institutes related
  • Creador: Departamento: Sistemas y Recursos Naturales
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