|
Descripción
|
|
|---|---|
| Expressed in somatosensory neurons of the dorsal root and trigeminal ganglion, the transient receptor potential melastatin 8 (TRPM8) channel is a Ca(2+)-permeable cation channel activated by cold, voltage, phosphatidylinositol 4,5-bisphosphate, and menthol. Although TRPM8 channel gating has been characterized at the single channel and macroscopic current levels, there is currently no consensus regarding the extent to which temperature and voltage sensors couple to the conduction gate. In this study, we extended the range of voltages where TRPM8-induced ionic currents were measured and made careful measurements of the maximum open probability the channel can attain at different temperatures by means of fluctuation analysis. The first direct measurements of TRPM8 channel temperature-driven conformational rearrangements provided here suggest that temperature alone is able to open the channel and that the opening reaction is voltage-independent. Voltage is a partial activator of TRPM8 channels, because absolute open probability values measured with fully activated voltage sensors are less than 1, and they decrease as temperature rises. By unveiling the fast temperature-dependent deactivation process, we show that TRPM8 channel deactivation is well described by a double exponential time course. The fast and slow deactivation processes are temperature-dependent with enthalpy changes of 27.2 and 30.8 kcal mol(-1). The overall Q10 for the closing reaction is about 33. A three-tiered allosteric model containing four voltage sensors and four temperature sensors can account for the complex deactivation kinetics and coupling between voltage and temperature sensor activation and channel opening. | |
|
Internacional
|
Si |
|
JCR del ISI
|
Si |
|
Título de la revista
|
The Journal of Biological Chemistry |
|
ISSN
|
0021-9258 |
|
Factor de impacto JCR
|
4,6 |
|
Información de impacto
|
For 2013, the journal JOURNAL OF BIOLOGICAL CHEMISTRY has an Impact Factor of 4.600 |
|
Volumen
|
289 |
|
DOI
|
10.1074/jbc.m114.612713 |
|
Número de revista
|
51 |
|
Desde la página
|
35438 |
|
Hasta la página
|
35454 |
|
Mes
|
DICIEMBRE |
|
Ranking
|
Category Name: BIOCHEMISTRY & MOLECULAR BIOLOGY Total Journals in Category: 291 Journal Rank in Category: 65 Quartile in Category: Q1 |