Observatorio de I+D+i UPM

Memorias de investigación
Research Publications in journals:
Temperature and Voltage Coupling to Channel Opening in Transient Receptor Potential Melastatin 8 (TRPM8)
Year:2014
Research Areas
  • Neurophysiology,
  • Structural biology,
  • Cell biology,
  • Molecular biology,
  • Biochemistry
Information
Abstract
Expressed in somatosensory neurons of the dorsal root and trigeminal ganglion, the transient receptor potential melastatin 8 (TRPM8) channel is a Ca(2+)-permeable cation channel activated by cold, voltage, phosphatidylinositol 4,5-bisphosphate, and menthol. Although TRPM8 channel gating has been characterized at the single channel and macroscopic current levels, there is currently no consensus regarding the extent to which temperature and voltage sensors couple to the conduction gate. In this study, we extended the range of voltages where TRPM8-induced ionic currents were measured and made careful measurements of the maximum open probability the channel can attain at different temperatures by means of fluctuation analysis. The first direct measurements of TRPM8 channel temperature-driven conformational rearrangements provided here suggest that temperature alone is able to open the channel and that the opening reaction is voltage-independent. Voltage is a partial activator of TRPM8 channels, because absolute open probability values measured with fully activated voltage sensors are less than 1, and they decrease as temperature rises. By unveiling the fast temperature-dependent deactivation process, we show that TRPM8 channel deactivation is well described by a double exponential time course. The fast and slow deactivation processes are temperature-dependent with enthalpy changes of 27.2 and 30.8 kcal mol(-1). The overall Q10 for the closing reaction is about 33. A three-tiered allosteric model containing four voltage sensors and four temperature sensors can account for the complex deactivation kinetics and coupling between voltage and temperature sensor activation and channel opening.
International
Si
JCR
Si
Title
The Journal of Biological Chemistry
ISBN
0021-9258
Impact factor JCR
4,6
Impact info
For 2013, the journal JOURNAL OF BIOLOGICAL CHEMISTRY has an Impact Factor of 4.600
Volume
289
10.1074/jbc.m114.612713
Journal number
51
From page
35438
To page
35454
Month
DICIEMBRE
Ranking
Category Name: BIOCHEMISTRY & MOLECULAR BIOLOGY Total Journals in Category: 291 Journal Rank in Category: 65 Quartile in Category: Q1
Participants
  • Autor: Natalia Raddatz . (UPM)
  • Autor: Juan Pablo Castillo (Centro Interdisciplinario de Neurociencia de Valparaíso, Facultad de Ciencias, Universidad de Valparaíso)
  • Autor: Carlos González (Centro Interdisciplinario de Neurociencia de Valparaíso, Facultad de Ciencias, Universidad de Valparaíso)
  • Autor: Osvaldo Alvarez (Centro Interdisciplinario de Neurociencia de Valparaíso, Facultad de Ciencias, Universidad de Valparaíso and Departamento de Biología, Facultad de Ciencias, Universidad de Chile)
  • Autor: Ramón Latorre (Centro Interdisciplinario de Neurociencia de Valparaíso, Facultad de Ciencias, Universidad de Valparaíso)
Research Group, Departaments and Institutes related
  • Creador: Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología - Biología Vegetal
S2i 2019 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
Cofinanciación del MINECO en el marco del Programa INNPACTO (IPT-020000-2010-22)