Observatorio de I+D+i UPM

Memorias de investigación
Book chapters:
Functional genomics of symbiotic nitrogen fixation in legumes with a focus on transcription factors and membrane transporters
Research Areas
  • Natural sciences and health sciences
Transcription factors (TFs), transcription regulators (TR), and membrane transporters are major determinants of nodule development and function. TFs and TRs control genetic reprogramming, and transporters facilitate exchange of nutrients between plant and rhizobia among other things. Here, we describe the strategy of reverse genetic screening in Medicago truncatula for genes from these two functional classes. We employed well-established transcriptomic resources (Medicago gene expression atlas), a spatially resolved nodule transcriptomic data set, and the tobacco retrotransposon Tnt1 insertion mutant population for systematic selection and functional analysis of candidate genes. We also re-annotated 417 nodule-induced TF and TR genes according to IMGAG3.5v4, which is the latest release of the Medicago genome. In total, we have targeted 21 nodule-enhanced TF and TR genes, and 20 nodule-enhanced transporter genes for characterization via insertional mutagenesis. In this chapter, we emphasize the importance of TFs, TRs, and transport proteins for symbiotic nitrogen fixation (SNF) and discuss the use of the Medicago Tnt1 mutant population for studies of gene function.
Book Edition
Book Publishing
2015 John Wiley & Sons, Inc
Book title
Biological Nitrogen Fixation
From page
To page
  • Autor: Senjuti Sinharoy (Noble Foundation)
  • Autor: Igor Kryvoruchko (Noble Foundation)
  • Autor: Catalina I. Pislariu (Noble Foundation)
  • Autor: Manuel Gonzalez Guerrero (UPM)
  • Autor: Vagner A. Benedito (West Virginia University)
  • Autor: Michael K. Udvardi (Noble Foundation)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: BIOLOGÍA MOLECULAR Y COMPUTACIONAL
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología - Biología Vegetal
S2i 2020 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
Cofinanciación del MINECO en el marco del Programa INNPACTO (IPT-020000-2010-22)