Observatorio de I+D+i UPM

Memorias de investigación
Communications at congresses:
Symbiosis of the endangered Lupinus mariae-josephae lupin especies: Successful ?in situ? propagation with rhizobial inoculation
Year:2015
Research Areas
  • Natural sciences and health sciences
Information
Abstract
region, in Eastern Spain. This lupine thrives in alkaline soils with high pH, a unique habitat for lupines. In these soils, Lmj grows in just a few defined patches, and previous conservation efforts directed towards controlled plant reproduction have been unsuccessful. A legislative decree (70/2009, page 20156 Anex I) published in the el 'Diario Oficial de la Comunitat Valenciana' shows Lmj in a category corresponding, in the latest version of the Red List of IUCN (IUCN, 2012) (International Union for Conservation of Nature and Nature Resources), to an ?Endangered? legume species not extinct in the wild. Most current IUCN criteria used to define rare, small-range legume species, are based on history of reproductive traits such as number of pods and seeds. We have previously shown that Lmj plants establish a specific root nodule symbiosis with bradyrhizobia present in those soils, and we reasoned that the paucity of these bacteria in soils might contribute to the lack of success in reproducing plants for conservation purposes. Greenhouse experiments using Lmj trap-plants showed an absence, or very low concentration, of Lmj-nodulating bacteria in ?terra rossa? soils of Valencia outside of Lmj plant patches. No Lmj endosymbiotic bacteria were found in ?terra rossa? or alkaline red soils outside the Valencia Lmj endemism region in the Iberian Peninsula or Balearic Islands. Among the rhizobia able to establish an efficient symbiosis with L. mariae-josephae plants, two Bradyrhizobium sp. strains, LmjC and LmjM3, were selected as inocula for seed coating. Two planting experiments were carried out in consecutive years under natural conditions in areas with edapho-climatic characteristics identical to those sustaining natural Lmj populations, and successful reproduction of the plant was achieved. Interestingly, the successful reproductive cycle was absolutely dependent on seedling inoculation with effective bradyrhizobia, and optimal performance was observed in plants inoculated with LmjC, a strain that had previously shown the most efficient behavior under controlled conditions. These results define conditions for L. mariae-josephae conservation and for extension to alkaline-limed soil habitats, where no other known lupine can thrive. Broadly speaking, the work singularly identified the rhizobial symbiosis as a factor affecting the conservation of legumes and often being exceedingly vulnerable to threats. Our results also indicate that seed inoculation with N2-fixing, efficient Rhizobium strains is a strategy to consider in the conservation of endangered legume species
International
Si
Congress
IV Congreso Luso-Español de Fijación de Nitrógeno y XV Reunión Nacional de Fijación de Nitrógeno de la SEFIN
960
Place
Leon
Reviewers
Si
ISBN/ISSN
0000000000
Start Date
16/06/2015
End Date
18/06/2015
From page
77
To page
77
IV Congreso Luso-Español de Fijación de Nitrógeno y XV Reunión Nacional de Fijación de Nitrógeno de la SEFIN
Participants
  • Autor: David Ricardo Duran Wendt (UPM)
  • Autor: A. Navarro (Centro para la Investigacion y Experimentación Forestal (CIEF). Generalitat Valenciana)
  • Autor: Luis Rey Navarro (UPM)
  • Autor: Juan Imperial Ródenas (UPM)
  • Autor: Tomas-Andres Ruiz Argueso (UPM)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: BIOLOGÍA MOLECULAR Y COMPUTACIONAL
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología - Biología Vegetal
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