Memorias de investigación
Ponencias en congresos:
Genomic analysis of three Bradyrhizobium geno(species) nodulating Lima bean (Phaseolus lunatus L.) in Peru
Año:2015
Áreas de investigación
  • Ciencias naturales y ciencias de la salud
Datos
Descripción
The Lima bean (Phaseolus lunatus), also known as pallar, ibes, garrofón or butter bean in Peru, México, Spain and USA, respectively, is the second most economically important species of Phaseolus. Peru is a centre of origin and domestication of Lima bean. This crop is cultivated mainly in the Central coast of Peru under a subtropical arid climate. In contrast to the common bean (Phaseolus vulgaris) which forms nodules with fast growing Rhizobium strains, the Lima bean forms nodules with slow growing bacteria of the Bradyrhizobium genus (López-López et al. 2013, Ormeño-Orrillo et al. 2006). We found strains of Bradyrhizobium yuanmingense and of three novel Bradyrhizobium genospecies inside P. lunatus nodules in Peru (Ormeño- Orrillo et al. 2006). Strains of the three novel genospecies were characterized by showing an extra-slow growing phenotype (generation time > 10 h-1) and strong alkali production in yeast extract mannitol medium. Two of the novel genospecies were recently named as Bradyrhizobium paxllaeri and Bradyrhizobium icense (Durán et al. 2014). B. paxllaeri strains dominate nodule occupancy followed by those of B. icense and then the third and yet-unnamed genospecies. With the aim to gain insights into this differential competitive ability, we sequenced the genome of one representative strain of each species. Sequencing was performed with the Illumina HiSeq or MiSeq platform and genome assembly with the SPAdes program. Gene prediction and automated annotation was performed with Prokka and RAST. Annotation of genes putatively involved in competitiveness was manually curated. Assemblies had from 55 to 175 contigs, with N50 sizes > 131 kb. Genome sizes of B. paxllaeri and B. icense were similar (8.2 Mb) and larger than that of the third genospecies (7.8 Mb). Preliminary analysis revealed differences between B. paxllaeri and the other two genospecies such as more genes for type IV pilus and two nodA genes. A comparative genomic analysis of P. lunatus symbionts will be presented at the meeting.
Internacional
Si
Nombre congreso
IV Congreso Luso-Español de Fijación de Nitrógeno y XV Reunión Nacional de Fijación de Nitrógeno de la SEFIN
Tipo de participación
960
Lugar del congreso
León
Revisores
Si
ISBN o ISSN
0000000000
DOI
Fecha inicio congreso
16/06/2015
Fecha fin congreso
18/06/2015
Desde la página
34
Hasta la página
34
Título de las actas
IV Congreso Luso-Español de Fijación de Nitrógeno y XV Reunión Nacional de Fijación de Nitrógeno de la SEFIN
Esta actividad pertenece a memorias de investigación
Participantes
  • Autor: E. Ormeño-Orrillo Universidad Nacional Agraria La Molina, Universidad Nacional Autónoma de México
  • Autor: D Zúñiga-Dávila Universidad Nacional Autónoma de México
  • Autor: Luis Rey Navarro UPM
  • Autor: David Ricardo Duran Wendt UPM
  • Autor: Juan Imperial Ródenas UPM
  • Autor: Tomas-Andres Ruiz Argueso UPM
  • Autor: Esperanza Martínez-Romero Universidad Nacional Autónoma de México
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Grupo de Investigación: BIOLOGÍA MOLECULAR Y COMPUTACIONAL
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología - Biología Vegetal