Observatorio de I+D+i UPM

Memorias de investigación
Artículos en revistas:
VPS13D Gene Variant Is Associated with Altered IL-6 Production and Mortality in Septic Shock
Año:2015
Áreas de investigación
  • Ciencias naturales y ciencias de la salud
Datos
Descripción
Genetic variations contribute to septic shock mortality. To discover a novel locus, we performed in vitro genome-wide association studies (GWAS) and further tested the result in a cohort of septic shock patients. Methods: Two in vitro GWAS using a quantitative trait locus analysis of stimulated IL-6 production in lymphoblastoid cells from 60 individuals of European ancestry were performed. VPS13D rs6685273 was genotyped in European ancestry patients (n = 498). The VPS13D gene was silenced in vitro. Results: Two GWAS using lymphoblastoid cells identified the locus of VPS13D rs6685273 that was significant in the same direction in both GWAS. The VPS13D rs6685273 C allele was associated with increased IL-6 production. Patients with septic shock who had the VPS13D rs6685273 CC genotype had an increased 28-day mortality (p = 0.023) and more organ failure (p < 0.05) compared to the CT/TT genotypes. VPS13D in vitro gene silencing in the HeLa cell line increased IL-6 production. Furthermore, the rs6685273 genotype was associated with differential VPS13D splice variant expression. Conclusions: The VPS13D rs6685273 C allele was associated with increased IL-6 production in vitro. The patients with the VPS13D rs6685273 CC genotype had increased 28-day mortality and increased organ failure. VPS13D appears to regulate IL-6 production
Internacional
Si
JCR del ISI
Si
Título de la revista
Journal of Innate Immunity
ISSN
1662-811X
Factor de impacto JCR
4,557
Información de impacto
Volumen
7
DOI
Número de revista
Desde la página
545
Hasta la página
553
Mes
SIN MES
Ranking
Esta actividad pertenece a memorias de investigación
Participantes
  • Autor: Taka-aki Nakada (University of British Columbia)
  • Autor: John H. Boyd (University of British Columbia)
  • Autor: James A. Russell (University of British Columbia)
  • Autor: Rosalía Aguirre-Hernández (University of British Columbia)
  • Autor: Mark Denis Wilkinson (UPM)
  • Autor: Simone A. Thair (University of British Columbia)
  • Autor: Emiri Nakada (University of British Columbia)
  • Autor: Melissa K. McConechy (University of British Columbia)
  • Autor: Christopher D. Fjell (University of British Columbia)
  • Autor: Keith R. Walley (University of British Columbia)
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Grupo de Investigación: BIOLOGÍA MOLECULAR Y COMPUTACIONAL
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología - Biología Vegetal
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