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Descripción
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| Many species of animals and culture models have been used to study corneal wound healing. Despite their benefits and limitations, no models that resemble the physiological response to injury in humans have been developed using in vitro stratified epithelia. The aim of this study was to develop a reproducible in vitro wound healing model using stratified human corneal-limbal epithelial (HCLE) cells. Cell Culture: Telomerase-immortalized human corneal keratinocytes were grown in keratinocyte serum-free medium (K-SFM) (Life Technologies) to confluence (monolayer). Subsequently, cells were incubated for 7 days in Dulbecco?s modified Eagle?s medium (DMEM)/F-12 (Sigma-Aldrich) supplemented with 10% calf serum and 10 ng/ml epidermal growth factor to promote differentiation and stratification. After wounding, different cell culture media (K-SFM and DMEM/F-12) with or without 10% New-Born Calf Serum were used. | |
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Internacional
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Si |
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Nombre congreso
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ARVO The Association for Research in Vision and Ophthalmology 2015 Annual Meeting |
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Tipo de participación
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960 |
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Lugar del congreso
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Colorado Convention Center (CCC). Denver, CO 80202 |
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Revisores
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Si |
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ISBN o ISSN
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1552-5783 |
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DOI
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Fecha inicio congreso
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03/05/2015 |
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Fecha fin congreso
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07/05/2015 |
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Desde la página
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32 |
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Hasta la página
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40 |
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Título de las actas
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IOVS. ARVO Annual Meeting Abstract. |