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Descripción
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| The purpose of this study was to generate an in vitro wound healing model based on stratified human corneal-limbal epithelial (HCLE) cells that resembles the native corneal epithelium. Immortalized HCLE cells were grown to confluence in serum-free medium and switched to 10% serum to promote differentiation and stratification. Afterwards, a mechanical injury with a trephine, combined with epithelial debridement, was performed. Cells were grown in culture media DMEM/F12 supplemented with EGF, with or without serum. The effect of serum on the wound healing response was evaluated using phase-contrast microscopy and a barrier function (rose bengal uptake) assay. | |
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Internacional
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Si |
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JCR del ISI
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Si |
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Título de la revista
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European Journal of Ophthalmology |
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ISSN
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1120-6721 |
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Factor de impacto JCR
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1,068 |
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Información de impacto
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Volumen
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25 |
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DOI
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Número de revista
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6 |
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Desde la página
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250 |
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Hasta la página
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258 |
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Mes
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NOVIEMBRE |
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Ranking
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37 |