Observatorio de I+D+i UPM

Memorias de investigación
Communications at congresses:
New insights on winter-associated regulators of the circadian clock in poplar.
Year:2015
Research Areas
  • Natural sciences and health sciences,
  • Earth sciences,
  • Molecular, cellular and genetic biology,
  • Biological sciences,
  • Biology,
  • Genetics,
  • Molecular genetics,
  • Biological engineering,
  • Genetic engineering,
  • Genetically modified organisms,
  • Plants and animal biology and ecology
Information
Abstract
Winter dormancy is an adaptive mechanism that allows trees from temperate and cold regions to survive the harsh conditions of this season. This process is strongly influenced by daylength and temperature, which are signals that are integrated by the circadian clock. Low temperatures cause the disruption of the circadian clock in trees, which consists in a transcriptional activation followed by an arrhythmic expression. In this work we uncover winter-associated regulators of the circadian clock in poplar. We generated transgenic poplars that express a transcriptional fusion between the promoter of a circadian clock gene and the luciferase gene. Setting up a circadian luminiscence assay by which we register the luciferase activity from leaf discs with a luminometer, we have characterized the expression of this promoter under different photoperiods. Then, we carried out a Yeast One Hybrid (Y1H) screening with a library enriched in winter-associated factors using this promoter as bait. Candidate regulators are tested in vivo by transient assays in poplar, overexpressing and silencing the candidate genes. Our results indicate the selected promoter region contains the circadian elements as well as the luciferase activity shows the expected expression under both long and short days. In the Y1H screening, we found several candidates that are classified either as transcription factors or chromatin remodelers. We discuss the putative role of these proteins as regulators of the poplar circadian clock.
International
No
Congress
XXI REUNIÓN DE LA SOCIEDAD ESPAÑOLA DE FISIOLOGÍA VEGETAL
970
Place
Toledo, España
Reviewers
Si
ISBN/ISSN
978-84-606-8883-9
Start Date
14/06/2015
End Date
17/06/2015
From page
344
To page
344
New insights on winter-associated regulators of the circadian clock in poplar.
Participants
  • Autor: Jose Manuel Ramos Sanchez (UPM)
  • Autor: Alicia Moreno Cortes (UPM)
  • Autor: Daniel Conde Rodriguez (UPM)
  • Autor: Tamara Hernández-Verdeja (umea plant science centre)
  • Autor: Mariano Manuel Perales (UPM)
  • Autor: Isabel Marta Allona Alberich (UPM)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Biotecnología Vegetal
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología - Biología Vegetal
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