Observatorio de I+D+i UPM

Memorias de investigación
Communications at congresses:
Optimizing seed conservation protocols and cryopreservation at the CRF-INIA genebank to reduce genetic erosion
Year:2015
Research Areas
  • Plants and animal biology and ecology,
  • Crop production
Information
Abstract
The CRF-INIA is the Spanish National Centre for Plant Genetic Resources where a duplicate of all seed active collections belonging to the Spanish Network of the National PGR Program should be deposited. First samples date from 1980?s. Nowadays, the long term collection (base collection) comprises approximately 39,000 samples. The CRF-INIA genebank also maintains the largest active collection in Spain, with up to 22.000 accessions, meanly of cereals and grain legumes. At the CRF-INIA genebank the seed conservation protocol follows the FAO/IPGRI recommendations: seed desiccation at 13-15% RH and 20 °C, and storage at -4 or -18 °C, for the active and base collections, respectively. Seed viability monitoring is performed systematically through germination tests. For most species, the current conservation protocol shows good performance (Martín et al. 2014). However, in some plant groups there is an accumulation of samples with low longevity, for which sample regeneration is costly. For example, after 20 or 30 years of storage, some accessions of Brassica ssp., Lactuca sativa or Secale cereale, have shown low seed viability after storage. In some cases, samples with low germination rates might be not only due to low longevity but also to other reasons, such as problems with dormancy interfiering with the germination test performance and low initial quality of the samples. The general aim of this project is to find alternative storage protocols to reduce genetic erosion, present and future, of the conserved material at the CRF-INIA seedbank. Cryoconservation of seeds at different water contents are being tested in species with low seed longevity and of problematic regeneration (rye, onion and cabbage). Results would be compared to the conventional storage at -18 °C after medium/long term storage.
International
Si
Congress
Seed Longevity Workshop of the International Society for Seed Science (ISSS)
970
Place
Wernigerode (Alemania)
Reviewers
Si
ISBN/ISSN
0000000000000
Start Date
05/07/2015
End Date
08/07/2015
From page
66
To page
66
Seeds for future generations ? Determinants of longevity
Participants
  • Autor: Sara Mira Perez (UPM)
  • Autor: Felix Perez Garcia (UPM)
  • Autor: Isaura Martín (CRF_INIA)
  • Autor: M. Elena Gonzalez Benito (UPM)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: PLANGER Germoplasma Vegetal
  • Departamento: Biotecnología - Biología Vegetal
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